Ectopic expression

Ectopic expression is the expression of a gene in a tissue where it is not normally expressed.[1] Ectopic expression also refers to the expression of a gene at a point in the cell cycle or in an organism's development when it is not normally expressed.[2] This can be caused by a disease, or it can be artificially produced as a way to help determine what the function of that gene is.

Ectopic expression of a gene can be done by introducing a transgene with a modified promoter into the target organism (transient or stable transfection) or by using the Gal4-UAS system. Ectopic expression is used to gain further understanding of the gene's functions.

References

  1. ↑ Schepers, Ute (2006-03-06). RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C. elegans, Drosophila, and Mammals. John Wiley & Sons. ISBN 9783527604371.
  2. ↑ Prelich, G (March 2012). "Gene Overexpression: uses, mechanisms, and interpretation". Genetics. 190: 841–54. doi:10.1534/genetics.111.136911. PMID 22419077. Retrieved September 26, 2015.


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