FuGENE
FuGENE® is the name given to a series of non-liposomal transfection reagents produced by Fugent LLC[1] of Wisconsin, USA, and marketed globally by Promega. They are used to facilitate the insertion of RNA or plasmid DNA into cultured cells.
Currently marketed are FuGENE® 6 which has been used for many years in cell biology research, appearing in over 10,000 publications, and FuGENE® HD which is intended for hard-to-transfect cell lines.[2] FuGENE® HD is utilized in a broad range of research and pharmaceutical areas from stem cell therapy[3] to lentiviral production[4] as well as CRISPR.[5]
See also
References
- ↑ "Contact Us - Fugent LLC". Fugent LLC. Retrieved 2016-06-14.
- ↑ "FuGENE Transfection Reagents". Promega. Retrieved 17 April 2013.
- ↑ Jiang, Yongkang; Shi, Yuan; He, Jing; Zhang, Zhiyong; Zhou, Guangdong; Zhang, Wenjie; Cao, Yilin; Liu, Wei (2016-01-01). "Enhanced tenogenic differentiation and tendon-like tissue formation by tenomodulin overexpression in murine mesenchymal stem cells". Journal of Tissue Engineering and Regenerative Medicine: n/a–n/a. doi:10.1002/term.2150. ISSN 1932-7005.
- ↑ "BioTechniques - Lentiviral Production Using FuGENE® HD Transfection Reagent". www.biotechniques.com. Retrieved 2016-06-14.
- ↑ Cheng, Albert W.; Wang, Haoyi; Yang, Hui; Shi, Linyu; Katz, Yarden; Theunissen, Thorold W.; Rangarajan, Sudharshan; Shivalila, Chikdu S.; Dadon, Daniel B. (2013-10-01). "Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system". Cell Research. 23 (10): 1163–1171. doi:10.1038/cr.2013.122. ISSN 1001-0602. PMC 3790238
. PMID 23979020.
- Arnold AS, et al. (2006). "Comparing reagents for efficient transfection of human primary myoblasts: FuGENE 6, Effectene and ExGen 500.". Fundamental Clinical Pharmacology. 20(1): 81–9. doi:10.1111/j.1472-8206.2005.00344.x.
- Yamano S, et al. (2010). "Comparison of transfection efficiency of nonviral gene transfer reagents.". Molecular Biotechnology. 46(3): 287–300. doi:10.1007/s12033-010-9302-5.
External links
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