Metal-coded affinity tag

Metal-coded affinity tag is a method used for quantitative proteomics by mass spectrometry that uses a metal chelate complex 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (DOTA) coupled to different lanthanide ions.^[1]^[2] The metal complexes attach to the cysteine residues of proteins in a sample.

Proteomic analysis

For bottom-up proteomics, the proteins can be separated by two-dimensional gel electrophoresis and analyzed by matrix-assisted laser desorption/ionization (MALDI) or electrospray ionization mass spectrometry for relative quantification or by inductively coupled plasma mass spectrometry for absolute quantification. For top-down proteomics, the undigested labeled proteins are analyzed.

↑ Ahrends, R.; Pieper, S.; Kuhn, A.; Weisshoff, H.; Hamester, M.; Lindemann, T.; Scheler, C.; Lehmann, K.; Taubner, K.; Linscheid, M. W. (2007). "A Metal-coded Affinity Tag Approach to Quantitative Proteomics". Molecular & Cellular Proteomics. 6 (11): 1907–1916. doi:10.1074/mcp.M700152-MCP200. ISSN 1535-9476. PMID 17627934.
↑ Messana, Irene; Cabras, Tiziana; Iavarone, Federica; Vincenzoni, Federica; Urbani, Andrea; Castagnola, Massimo (2013). "Unraveling the different proteomic platforms". Journal of Separation Science. 36 (1): 128–139. doi:10.1002/jssc.201200830. ISSN 1615-9306.

Quantitative proteomics

Protein mass spectrometry Proteomics Mass spectrometry

Quantification	Label-free quantification Stable isotope labeling by/with amino acids in cell culture (SILAC) Isobaric labeling Tandem mass tags (TMT) Isobaric tags for relative and absolute quantitation (iTRAQ) Isotope-coded affinity tags (ICAT) Metal-coded affinity tag (MeCAT) N-terminal labeling

Acquisition methods	Shotgun proteomics Targeted proteomics / SRM Data-independent acquisition

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