PrfA thermoregulator UTR
PrfA thermoregulator UTR | |
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Predicted secondary structure and sequence conservation of PrfA | |
Identifiers | |
Symbol | PrfA |
Rfam | RF00038 |
Other data | |
RNA type | Cis-reg; thermoregulator |
Domain(s) | Bacteria |
SO | 0000233 |
The PrfA thermoregulator UTR is an RNA thermometer found in the 5' UTR of the prfA gene.[1] In Listeria monocytogenes, virulence genes are maximally expressed at 37 °C (human body temperature) but are almost silent at 30 °C.[2] The genes are controlled by PrfA, a transcriptional activator whose expression is thermoregulated.[3] It has been shown that the untranslated mRNA (UTR) preceding prfA, forms a secondary structure, which masks the ribosome binding region.[1] It is thought that at 37 °C, the hairpin structure 'melts' and the SD sequence is unmasked.[1]
References
- 1 2 3 Johansson J, Mandin P, Renzoni A, Chiaruttini C, Springer M, Cossart P (2002). "An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes". Cell. 110 (5): 551–61. doi:10.1016/S0092-8674(02)00905-4. PMID 12230973.
- ↑ Freitag, NE; Port, GC; Miner, MD (September 2009). "Listeria monocytogenes - from saprophyte to intracellular pathogen.". Nature reviews. Microbiology. 7 (9): 623–8. doi:10.1038/nrmicro2171. PMC 2813567
. PMID 19648949.
- ↑ Digel, I; Kayser, P; Artmann, GM (2008). "Molecular processes in biological thermosensation.". Journal of biophysics (Hindawi Publishing Corporation : Online). 2008: 602870. doi:10.1155/2008/602870. PMC 2814129
. PMID 20130806.
External links
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