Schneider 2 cells
Schneider 2 cells, usually abbreviated as S2 cells, are one of the most commonly used Drosophila melanogaster cell lines. S2 cells were derived from a primary culture of late stage (20–24 hours old) Drosophila melanogaster embryos,[1] likely from a macrophage-like lineage.
S2 cells can be grown at room temperature both as a semi-adherent monolayer or in suspension, and they can be grown in the absence of serum.[2]
Several media have been developed for culturing insect cell lines with many of them suitable for culturing S2 cells. The S2 cells have been shown to grow up to 51.2×106 cells/ml in serum free medium[3] and above 106 cells/ml in basal media such as that used in Schneider's experiments.[4] ExpreS2ion Biotechnologies have shown a cell concentration of up 70x106 cell/ml.[5]
References
- ↑ Schneider I (1972). "Cell Lines Derived from Late Embryonic Stages of Drosophila melanogaster". J. Embryol. Exp. Morph. 27: 363–365.
- ↑ Invitrogen (05/02/2002). Drosophila Schneider 2 (S2) Cells (PDF). Version F. Invitrogen. 28-0172. Retrieved 2012-02-25. Check date values in:
|date=(help) - ↑ Pamboukian, Marilena Martins; Jorge, Soraia Athie Calil; Santos, Mariza Gerdulo; Yokomizo, Adriana Yurie; Pereira, Carlos Augusto; Tonso, Aldo (2008-02-27). "Insect cells respiratory activity in bioreactor". Cytotechnology. 57 (1): 37–44. doi:10.1007/s10616-007-9118-8. ISSN 0920-9069. PMC 2553646
. PMID 19003170. - ↑ Moraes, Ângela M.; Jorge, Soraia A. C.; Astray, Renato M.; Suazo, Claudio A. T.; Calderón Riquelme, Camilo E.; Augusto, Elisabeth F. P.; Tonso, Aldo; Pamboukian, Marilena M.; Piccoli, Rosane A. M. (2012-05-01). "Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development". Biotechnology Advances. 30 (3): 613–628. doi:10.1016/j.biotechadv.2011.10.009.
- ↑ Dyring, Charlotte. "Optimising the Drosophila S2 Expression System for Production of Therapeutic Vaccines". BioProcessing Journal. 10 (2): 28–35. doi:10.12665/j102.dyring.