AFO
AFO may refer to:
- Absolute Force Online, a video game
- Academia Film Olomouc
- Advanced Force Operations
- Aero Force One, a fan club for the rock band Aerosmith
- Afghan Film Organization
- Afo Dodoo (born 1973), Ghanaian footballer
- AFO Records, the first African-American owned record label in the United States
- Air Force One (disambiguation)
- Alien Front Online, a video game
- Animal Face-Off, a television show
- Animal feeding operation, as defined by the Environmental Protection Agency
- Anime Festival Orlando, a yearly anime convention in Orlando, Florida
- Ankle-foot orthosis, a brace for the ankle and foot
- Anti-Fascist Organisation, a provisional organization in Burma during World War II
- Archiv für Orientforschung, a scholarly journal
- Arellano-Felix Organization, a drug trafficking cartel in Mexico, also known as the Tijuana Cartel
- Association of Field Ornithologists, an ornithological organization, publishers of Journal of Field Ornithology
- Australian Field Ornithology, a journal of BirdLife Australia
- Authorised Firearms Officer, a police officer in the United Kingdom who has received training and authorization to carry and use firearms
- Eloyi language (ISO-639: afo)
- Eloyi people, also called Afo
- Francophone Assembly of Ontario (French: L'assemblée de la Francophonie de l'Ontario, AFO)
- Afo, thought to be the world's oldest Clove Tree
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