Biocatalysis

Biocatalysis (/ˌbaɪoʊkəˈtælᵻsᵻs/) is catalysis in living (biological) systems. In biocatalytic processes, natural catalysts, such as protein enzymes, perform chemical transformations on organic compounds. Both enzymes that have been more or less isolated and enzymes still residing inside living cells are employed for this task.[1][2][3]

History

Biocatalysis underpins some of the oldest chemical transformations known to humans, for brewing predates recorded history. The oldest records of brewing are about 6000 years old and refer to the Sumerians.

The employment of enzymes and whole cells have been important for many industries for centuries. The most obvious uses have been in the food and drink businesses where the production of wine, beer, cheese etc. is dependent on the effects of the microorganisms.

More than one hundred years ago, biocatalysis was employed to do chemical transformations on non-natural man-made organic compounds, with the last 30 years seeing a substantial increase in the application of biocatalysis to produce fine chemicals, especially for the pharmaceutical industry.[4]

Since biocatalysis deals with enzymes and microorganisms, it is historically classified separately from "homogeneous catalysis" and "heterogeneous catalysis". However, mechanistically speaking, biocatalysis is simply a special case of heterogeneous catalysis.[5]

Advantages

A key concept in organic synthesis is selectivity, the ability to work with a single compound, which is necessary to obtain a high yield of a specific product. There are a large range of selective organic reactions available for most synthetic needs. However, there is still one area where organic chemists are struggling, and that is when chirality is involved, although considerable progress in chiral synthesis has been achieved in recent years.

Enzymes display three major types of selectivities:

These reasons, and especially the latter, are the major reasons why synthetic chemists have become interested in biocatalysis. This interest in turn is mainly due to the need to synthesise enantiopure compounds as chiral building blocks for Pharmaceutical drugs and agrochemicals.

Reaction similarity

Similarity between enzymatic reactions (EC) can be calculated by using bond changes, reaction centres or substructure metrics (EC-BLAST).[6]

Another important advantage of biocatalysts are that they are environmentally acceptable, being completely degraded in the environment. Furthermore, the enzymes act under mild conditions, which minimizes problems of undesired side-reactions such as decomposition, isomerization, racemization and rearrangement, which often plague traditional methodology.

Immobilized enzymes demonstrate very high stability and re-usability and can be used to conduct reactions in continuous mode in microreactors.[7][8]

Asymmetric biocatalysis

The use of biocatalysis to obtain enantiopure compounds can be divided into two different methods:

  1. Kinetic resolution of a racemic mixture
  2. Biocatalysed asymmetric synthesis

In kinetic resolution of a racemic mixture, the presence of a chiral object (the enzyme) converts one of the stereoisomers of the reactant into its product at a greater reaction rate than for the other reactant stereoisomer.

The stereochemical mixture has now been transformed into a mixture of two different compounds, making them separable by normal methodology. The maximum yield in such kinetic resolutions is 50%, since a yield of more than 50% means that some of wrong isomer also has reacted, giving a lower enantiomeric excess. Such reactions must therefore be terminated before equilibrium is reached. If it is possible to perform such resolutions under conditions where the two substrate- enantiomers are racemizing continuously, all substrate may in theory be converted into enantiopure product. This is called dynamic resolution.

In biocatalysed asymmetric synthesis, a non-chiral unit becomes chiral in such a way that the different possible stereoisomers are formed in different quantities. The chirality is introduced into the substrate by influence of enzyme, which is chiral. Yeast is a biocatalyst for the enantioselective reduction of ketones.

The Baeyer–Villiger oxidation is another example of a biocatalytic reaction. In one study a specially designed mutant of Candida antarctica was found to be an effective catalyst for the Michael addition of acrolein with acetylacetone at 20 °C in absence of additional solvent.[9]

Another study demonstrates how racemic nicotine (mixture of S and R-enantiomers 1 in scheme 3) can be deracemized in a one-pot procedure involving a monoamine oxidase isolated from Aspergillus niger which is able to oxidize only the amine S-enantiomer to the imine 2 and involving an ammonia–borane reducing couple which can reduce the imine 2 back to the amine 1.[10] In this way the S-enantiomer will continuously be consumed by the enzyme while the R-enantiomer accumulates. It is even possible to stereoinvert pure S to pure R.

References

  1. ↑ Anthonsen, Thorlief (2000). "Reactions Catalyzed by Enzymes". In Adlercreutz, Patrick; Straathof, Adrie J. J. Applied Biocatalysis (2nd ed.). Taylor & Francis. pp. 18–59. ISBN 9058230244. Retrieved 9 February 2013.
  2. ↑ Faber, Kurt (2011). Biotransformations in Organic Chemistry (6th ed.). Springer. ISBN 9783642173936.
  3. ↑ Jayasinghe, Leonard Y.; Smallridge, Andrew J.; Trewhella, Maurie A. (1993). "The yeast mediated reduction of ethyl acetoacetate in petroleum ether". Tetrahedron Letters. 34 (24): 3949–3950. doi:10.1016/S0040-4039(00)79272-0.
  4. ↑ Liese, Andreas; Seelbach, Karsten; Wandrey, Christian, eds. (2006). Industrial Biotransformations (2nd ed.). John Wiley & Sons. p. 556. ISBN 3527310010.
  5. ↑ Rothenberg, Gadi (2008). Catalysis: Concepts and green applications. Wiley. ISBN 9783527318247.
  6. ↑ Rahman, SA; Cuesta, SM; Furnham, N; Holliday, GL; Thornton, JM (2014). "EC-BLAST: a tool to automatically search and compare enzyme reactions". Nature Methods. 11: 171–174. doi:10.1038/nmeth.2803. PMID 24412978.
  7. ↑ Bhangale, Atul; Kathryn L. Beers; Richard A. Gross (2012). "Enzyme-Catalyzed Polymerization of End-Functionalized Polymers in a Microreactor". Macromolecules. 45: 7000–7008. doi:10.1021/ma301178k.
  8. ↑ Bhangale, Atul; Santanu Kundu; William E. Wallace; Kathleen M. Flynn; Charles M. Guttman; Richard A. Gross; Kathryn L. Beers (2010). "Continuous Flow Enzyme-Catalyzed Polymerization in a Microreactor". JACS. 133: 6006–6011. doi:10.1021/ja111346c.
  9. ↑ Svedendahl, Maria; Hult, Karl; Berglund, Per (December 2005). "Fast Carbon-Carbon Bond Formation by a Promiscuous Lipase". Journal of the American Chemical Society. 127 (51): 17988–17989. doi:10.1021/ja056660r. PMID 16366534.
  10. ↑ Dunsmore, Colin J.; Carr,Reuben; Fleming, Toni; Turner, Nicholas J. (2006). "A Chemo-Enzymatic Route to Enantiomerically Pure Cyclic Tertiary Amines". Journal of the American Chemical Society. 128 (7): 2224–2225. doi:10.1021/ja058536d. PMID 16478171.

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