CDC27

CDC27
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3T1N, 4RG6, 4RG7, 4RG9, 4UI9, 5A31, 5G04, 5G05
Identifiers
Aliases	CDC27, ANAPC3, APC3, CDC27Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2, cell division cycle 27
External IDs	MGI: 102685 HomoloGene: 960 GeneCards: CDC27
Gene ontology Molecular function • protein phosphatase binding • protein binding Cellular component • cytoplasm • cytosol • centrosome • spindle • spindle microtubule • anaphase-promoting complex • nucleus • nucleoplasm Biological process • metaphase/anaphase transition of mitotic cell cycle • negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle • anaphase-promoting complex-dependent catabolic process • cell proliferation • positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition • regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle • protein ubiquitination • protein K11-linked ubiquitination • protein ubiquitination involved in ubiquitin-dependent protein catabolic process • proteasome-mediated ubiquitin-dependent protein catabolic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	996	217232
Ensembl	ENSG00000004897	ENSMUSG00000020687
UniProt	P30260	A2A6Q5
RefSeq (mRNA)	NM_001114091 NM_001256 NM_001293089 NM_001293091	NM_001285988 NM_001285989 NM_001285990 NM_145436
RefSeq (protein)	NP_001107563.1 NP_001247.3 NP_001280018.1	NP_001272917.1 NP_663411.2
Location (UCSC)	Chr 17: 47.12 – 47.19 Mb	Chr 11: 104.5 – 104.55 Mb
PubMed search	[1]	[2]
Wikidata

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Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene.^[3][4]

Function

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.^[4]

Interactions

CDC27 has been shown to interact with:

• ANAPC10,^[5][6]
• ANAPC11,^[5][6]
• ANAPC1,^[5][7]
• ANAPC4,^[5][6]
• ANAPC5,^[5][6][8]
• ANAPC7,^[5][6]
• CDC16,^{[5][6][9][10]}
• CDC20,^{[5][9][11][12][13][14][15]}
• CDC23,^[5][6]
• CDH1,^[5]
• FZR1,^[11][16]
• MAD2L1^[9][14] and
• PIN1.^[17][18]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Tugendreich S, Boguski MS, Seldin MS, Hieter P (Nov 1993). "Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base". Proceedings of the National Academy of Sciences of the United States of America. 90 (21): 10031–5. doi:10.1073/pnas.90.21.10031. PMC 47707. PMID 8234252.
4. "Entrez Gene: CDC27 cell division cycle 27 homolog (S. cerevisiae)".
5. Vodermaier HC, Gieffers C, Maurer-Stroh S, Eisenhaber F, Peters JM (Sep 2003). "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Current Biology. 13 (17): 1459–68. doi:10.1016/s0960-9822(03)00581-5. PMID 12956947.
6. Gmachl M, Gieffers C, Podtelejnikov AV, Mann M, Peters JM (Aug 2000). "The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex". Proceedings of the National Academy of Sciences of the United States of America. 97 (16): 8973–8. doi:10.1073/pnas.97.16.8973. PMC 16806. PMID 10922056.
7. Sumara I, Vorlaufer E, Gieffers C, Peters BH, Peters JM (Nov 2000). "Characterization of vertebrate cohesin complexes and their regulation in prophase". The Journal of Cell Biology. 151 (4): 749–62. doi:10.1083/jcb.151.4.749. PMC 2169443. PMID 11076961.
8. Koloteva-Levine N, Pinchasi D, Pereman I, Zur A, Brandeis M, Elroy-Stein O (May 2004). "The Apc5 subunit of the anaphase-promoting complex/cyclosome interacts with poly(A) binding protein and represses internal ribosome entry site-mediated translation". Molecular and Cellular Biology. 24 (9): 3577–87. doi:10.1128/mcb.24.9.3577-3587.2004. PMC 387753. PMID 15082755.
9. Kallio M, Weinstein J, Daum JR, Burke DJ, Gorbsky GJ (Jun 1998). "Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events". The Journal of Cell Biology. 141 (6): 1393–406. doi:10.1083/jcb.141.6.1393. PMC 2132789. PMID 9628895.
10. Ollendorff V, Donoghue DJ (Dec 1997). "The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex". The Journal of Biological Chemistry. 272 (51): 32011–8. doi:10.1074/jbc.272.51.32011. PMID 9405394.
11. Kramer ER, Gieffers C, Hölzl G, Hengstschläger M, Peters JM (Nov 1998). "Activation of the human anaphase-promoting complex by proteins of the CDC20/Fizzy family". Current Biology. 8 (22): 1207–10. doi:10.1016/s0960-9822(07)00510-6. PMID 9811605.
12. D'Angiolella V, Mari C, Nocera D, Rametti L, Grieco D (Oct 2003). "The spindle checkpoint requires cyclin-dependent kinase activity". Genes & Development. 17 (20): 2520–5. doi:10.1101/gad.267603. PMC 218146. PMID 14561775.
13. Kallio MJ, Beardmore VA, Weinstein J, Gorbsky GJ (Sep 2002). "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". The Journal of Cell Biology. 158 (5): 841–7. doi:10.1083/jcb.200201135. PMC 2173153. PMID 12196507.
14. Wassmann K, Benezra R (Sep 1998). "Mad2 transiently associates with an APC/p55Cdc complex during mitosis". Proceedings of the National Academy of Sciences of the United States of America. 95 (19): 11193–8. doi:10.1073/pnas.95.19.11193. PMC 21618. PMID 9736712.
15. Nilsson J, Yekezare M, Minshull J, Pines J (Dec 2008). "The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction". Nature Cell Biology. 10 (12): 1411–20. doi:10.1038/ncb1799. PMC 2635557. PMID 18997788.
16. Zhou Y, Ching YP, Ng RW, Jin DY (Sep 2003). "Differential expression, localization and activity of two alternatively spliced isoforms of human APC regulator CDH1". The Biochemical Journal. 374 (Pt 2): 349–58. doi:10.1042/BJ20030600. PMC 1223613. PMID 12797865.
17. Shen M, Stukenberg PT, Kirschner MW, Lu KP (Mar 1998). "The essential mitotic peptidyl-prolyl isomerase Pin1 binds and regulates mitosis-specific phosphoproteins". Genes & Development. 12 (5): 706–20. doi:10.1101/gad.12.5.706. PMC 316589. PMID 9499405.
18. Lu PJ, Zhou XZ, Shen M, Lu KP (Feb 1999). "Function of WW domains as phosphoserine- or phosphothreonine-binding modules". Science. 283 (5406): 1325–8. doi:10.1126/science.283.5406.1325. PMID 10037602.

Further reading

• Tugendreich S, Tomkiel J, Earnshaw W, Hieter P (Apr 1995). "CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition". Cell. 81 (2): 261–8. doi:10.1016/0092-8674(95)90336-4. PMID 7736578.
• Chen PL, Ueng YC, Durfee T, Chen KC, Yang-Feng T, Lee WH (Feb 1995). "Identification of a human homologue of yeast nuc2 which interacts with the retinoblastoma protein in a specific manner". Cell Growth & Differentiation. 6 (2): 199–210. PMID 7756179.
• Ho PP, Couch FJ, Brody LC, Abel KJ, Boehnke M, Shearon TH, Chandrasekharappa SC, Collins FS, Weber BL (Sep 1995). "Localization of the human homolog of the yeast cell division control 27 gene (CDC27) proximal to ITGB3 on human chromosome 17q21.3". Somatic Cell and Molecular Genetics. 21 (5): 351–5. doi:10.1007/BF02257470. PMID 8619132.
• Ollendorff V, Donoghue DJ (Dec 1997). "The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex". The Journal of Biological Chemistry. 272 (51): 32011–8. doi:10.1074/jbc.272.51.32011. PMID 9405394.
• Yu H, Peters JM, King RW, Page AM, Hieter P, Kirschner MW (Feb 1998). "Identification of a cullin homology region in a subunit of the anaphase-promoting complex". Science. 279 (5354): 1219–22. doi:10.1126/science.279.5354.1219. PMID 9469815.
• Shen M, Stukenberg PT, Kirschner MW, Lu KP (Mar 1998). "The essential mitotic peptidyl-prolyl isomerase Pin1 binds and regulates mitosis-specific phosphoproteins". Genes & Development. 12 (5): 706–20. doi:10.1101/gad.12.5.706. PMC 316589. PMID 9499405.
• Zhou BB, Li H, Yuan J, Kirschner MW (Jun 1998). "Caspase-dependent activation of cyclin-dependent kinases during Fas-induced apoptosis in Jurkat cells". Proceedings of the National Academy of Sciences of the United States of America. 95 (12): 6785–90. doi:10.1073/pnas.95.12.6785. PMC 22635. PMID 9618490.
• Wassmann K, Benezra R (Sep 1998). "Mad2 transiently associates with an APC/p55Cdc complex during mitosis". Proceedings of the National Academy of Sciences of the United States of America. 95 (19): 11193–8. doi:10.1073/pnas.95.19.11193. PMC 21618. PMID 9736712.
• Lu PJ, Zhou XZ, Shen M, Lu KP (Feb 1999). "Function of WW domains as phosphoserine- or phosphothreonine-binding modules". Science. 283 (5406): 1325–8. doi:10.1126/science.283.5406.1325. PMID 10037602.
• Grossberger R, Gieffers C, Zachariae W, Podtelejnikov AV, Schleiffer A, Nasmyth K, Mann M, Peters JM (May 1999). "Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex". The Journal of Biological Chemistry. 274 (20): 14500–7. doi:10.1074/jbc.274.20.14500. PMID 10318877.
• Chan GK, Jablonski SA, Sudakin V, Hittle JC, Yen TJ (Sep 1999). "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC". The Journal of Cell Biology. 146 (5): 941–54. doi:10.1083/jcb.146.5.941. PMC 2169490. PMID 10477750.
• Gieffers C, Peters BH, Kramer ER, Dotti CG, Peters JM (Sep 1999). "Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons". Proceedings of the National Academy of Sciences of the United States of America. 96 (20): 11317–22. doi:10.1073/pnas.96.20.11317. PMC 18031. PMID 10500174.
• Gmachl M, Gieffers C, Podtelejnikov AV, Mann M, Peters JM (Aug 2000). "The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex". Proceedings of the National Academy of Sciences of the United States of America. 97 (16): 8973–8. doi:10.1073/pnas.97.16.8973. PMC 16806. PMID 10922056.
• Rippmann JF, Hobbie S, Daiber C, Guilliard B, Bauer M, Birk J, Nar H, Garin-Chesa P, Rettig WJ, Schnapp A (Jul 2000). "Phosphorylation-dependent proline isomerization catalyzed by Pin1 is essential for tumor cell survival and entry into mitosis". Cell Growth & Differentiation. 11 (7): 409–16. PMID 10939594.
• Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (Sep 2000). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Reports. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
• Wendt KS, Vodermaier HC, Jacob U, Gieffers C, Gmachl M, Peters JM, Huber R, Sondermann P (Sep 2001). "Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex". Nature Structural Biology. 8 (9): 784–8. doi:10.1038/nsb0901-784. PMID 11524682.
• Stroschein SL, Bonni S, Wrana JL, Luo K (Nov 2001). "Smad3 recruits the anaphase-promoting complex for ubiquitination and degradation of SnoN". Genes & Development. 15 (21): 2822–36. doi:10.1101/gad.912901. PMC 312804. PMID 11691834.
• Kallio MJ, Beardmore VA, Weinstein J, Gorbsky GJ (Sep 2002). "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". The Journal of Cell Biology. 158 (5): 841–7. doi:10.1083/jcb.200201135. PMC 2173153. PMID 12196507.
• Topper LM, Campbell MS, Tugendreich S, Daum JR, Burke DJ, Hieter P, Gorbsky GJ (2002). "The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis". Cell Cycle. 1 (4): 282–92. doi:10.4161/cc.1.4.139. PMID 12429948.