Guanidinium chloride
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| Names | |||
|---|---|---|---|
| IUPAC name
Carbamimidoylazanium chloride | |||
| Other names
Guanidine hydrochloride | |||
| Identifiers | |||
50-01-1  | |||
| 3D model (Jmol) | Interactive image | ||
| ChEBI | CHEBI:32735 | ||
| ChemSpider | 5540 | ||
| ECHA InfoCard | 100.000.003 | ||
| PubChem | 10855382 | ||
| UNII | 3YQC9ZY4YB | ||
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| Properties | |||
| CH6ClN3 | |||
| Molar mass | 95.53 g·mol−1 | ||
| Appearance | orthorhombic bipyramidal crystals | ||
| Density | 1.354 g/cm3 at 20 °C | ||
| Melting point | 182.3 °C (360.1 °F; 455.4 K) | ||
| very soluble in water and ethanol[1] | |||
| Hazards | |||
| Safety data sheet | External MSDS | ||
| Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |||
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| Infobox references | |||
Guanidinium chloride or guanidine hydrochloride, usually abbreviated GuHCl and sometimes GdnHCl or GdmCl, is the hydrochloride salt of guanidine.
Structure
Guanidinium chloride crystallizes in orthorhombic space group Pbca. The crystal structure consists of a network of guanidinium cations and chloride anions linked by N–H···Cl hydrogen bonds.[2]
Use in protein denaturation
Guanidinium chloride is a strong chaotrope and one of the strongest denaturants used in physiochemical studies of protein folding. At high concentrations of guanidinium chloride (e.g., 6 M), proteins lose their ordered structure, and they tend to become randomly coiled, i.e. they do not contain any residual structure. However, at concentrations in the millimolar range in vivo, guanidinium chloride has been shown to "cure" prion positive cells (i.e. cells exhibiting a prion positive phenotype revert to a prion negative phenotype). This is the result of inhibition of the Hsp104 chaperone protein known to play an important role in prion fiber fragmentation and propagation.[3][4][5]
Historical survey
Petrunkin and Petrunkin (1927, 1928) appear to be the first who studied the binding of GndCl to gelatin and a mixture of thermally denatured protein from brain extract. Greenstein (1938, 1939), however, appears to be the first to discover the high denaturing action of guanidinium halides and thiocyanates in following the liberation of sulfhydryl groups in ovalbumin and few other proteins as a function of salt concentration.[6]
Medical Uses
Guanidine hydrochloride is indicated for the reduction of the symptoms of muscle weakness and easy fatigability associated with Eaton-Lambert syndrome. It is not indicated for treating myasthenia gravis. It apparently acts by enhancing the release of acetylcholine following a nerve impulse. It also appears to slow the rates of depolarization and repolarization of muscle cell membranes. Initial dosage is usually between 10 and 15 mg/kg (5 to 7 mg/pound) of body weight per day in 3 or 4 divided doses. This dosage may be gradually increased to a total daily dosage of 35 mg/kg (16 mg/pound) of body weight per day or up to the development of side effects. Side effects include increased peristalsis and diarrhea. Fatal bone-marrow suppression, apparently dose related, can occur with guanidine.
References
- ↑ Lide, David R. (1998). Handbook of Chemistry and Physics (87 ed.). Boca Raton, FL: CRC Press. pp. 3–296. ISBN 0-8493-0594-2.
- ↑ Haas, D. J.; Harris, D. R.; Mills, H. H. (1965). "The crystal structure of guanidinium chloride". Acta Crystallogr. 19: 676–679. doi:10.1107/S0365110X65004085.
- ↑ Ferreira PC, Ness F, Edwards SR, Cox BS, Tuite MF (2001) The elimination of the yeast [PSI+] prion by guanidine hydrochloride is the result of Hsp104 inactivation. Mol Microbiol 40 (6):1357-1369.
- ↑ Ness F, Ferreira P, Cox BS, Tuite MF (2002) Guanidine hydrochloride inhibits the generation of prion "seeds" but not prion protein aggregation in yeast. Mol Cell Biol 22 (15):5593-5605.
- ↑ Eaglestone SS, Ruddock LW, Cox BS, Tuite MF (2000) Guanidine hydrochloride blocks a critical step in the propagation of the prion-like determinant [PSI(+)] of Saccharomyces cerevisiae. Proc Natl Acad Sci USA 97 (1):240-244.
- ↑ Lapange, Savo (1978). Physicochemical aspects of protein denaturation. New York: Wiley. ISBN 0-471-03409-6.