Janus Green B

Janus Green B^[1]

Names
IUPAC name 8-(4-Dimethylaminophenyl)diazenyl-N,N-diethyl-10-phenylphenazin-10-ium-2-amine chloride
Other names Diazin Green S Union Green B
Identifiers
CAS Number	2869-83-2
3D model (Jmol)	Interactive image
ChEBI	CHEBI:21184
ChemSpider	68607
ECHA InfoCard	100.018.814
EC Number	220-695-6
PubChem	76123
InChI InChI=1S/C30H31N6.ClH/c1-5-35(6-2)26-17-19-28-30(21-26)36(25-10-8-7-9-11-25)29-20-23(14-18-27(29)31-28)33-32-22-12-15-24(16-13-22)34(3)4;/h7-21H,5-6H2,1-4H3;1H/q+1;/p-1 Key: XXACTDWGHQXLGW-UHFFFAOYSA-M
SMILES CCN(CC)C1=CC2=[N+](C3=C(C=CC(=C3)N=NC4=CC=C(C=C4)N(C)C)N=C2C=C1)C5=CC=CC=C5.[Cl-]
Properties
Chemical formula	C30H31ClN6
Molar mass	511.06 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.^[2]

The indicator Janus Green B changes colour according to the amount of oxygen present.^[3] When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.

References

1. Janus Green B, at Sigma-Aldrich
2. Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula. Archiv für mikroskopische Anatomie 55:558-575(Also Tafel XXXII)
3. http://www.mrothery.co.uk/bio_web_prac/practicals/2anaerobic%20resp%20student.doc