NDC80

NDC80
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2IGP, 2VE7, 3IZ0
Identifiers
Aliases	NDC80, HEC, HEC1, HsHec1, KNTC2, TID3, hskinetochore complex component
External IDs	MGI: 1914302 HomoloGene: 38141 GeneCards: NDC80
Gene ontology Molecular function • structural constituent of cytoskeleton • protein binding • identical protein binding Cellular component • condensed chromosome kinetochore • cytosol • membrane • condensed nuclear chromosome outer kinetochore • chromosome • Ndc80 complex • chromosome, centromeric region • nucleus • kinetochore Biological process • mitotic nuclear division • attachment of mitotic spindle microtubules to kinetochore • chromosome segregation • mitotic spindle organization • cell division • establishment of mitotic spindle orientation • mitotic sister chromatid segregation • cell cycle • attachment of spindle microtubules to kinetochore • sister chromatid cohesion Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10403	67052
Ensembl	ENSG00000080986	ENSMUSG00000024056
UniProt	O14777	Q9D0F1
RefSeq (mRNA)	NM_006101	NM_023294
RefSeq (protein)	NP_006092.1	NP_075783.2
Location (UCSC)	Chr 18: 2.57 – 2.62 Mb	Chr 17: 71.5 – 71.53 Mb
PubMed search	[1]	[2]
Wikidata

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Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene.^[3][4][5]

Function

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]^[5]

Interactions

NDC80 has been shown to interact with MIS12,^[6][7] NEK2^[8][9] and PSMC2.^[9]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Chen Y, Riley DJ, Chen PL, Lee WH (Oct 1997). "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis". Molecular and Cellular Biology. 17 (10): 6049–56. doi:10.1128/mcb.17.10.6049. PMC 232454. PMID 9315664.
4. Martin-Lluesma S, Stucke VM, Nigg EA (Sep 2002). "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2". Science. 297 (5590): 2267–70. doi:10.1126/science.1075596. PMID 12351790.
5. "Entrez Gene: NDC80 NDC80 homolog, kinetochore complex component (S. cerevisiae)".
6. Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A (Sep 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes & Development. 18 (18): 2255–68. doi:10.1101/gad.1234104. PMC 517519. PMID 15371340.
7. Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology. 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821.
8. Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH (Dec 2002). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". The Journal of Biological Chemistry. 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167.
9. Chen Y, Sharp ZD, Lee WH (Sep 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". The Journal of Biological Chemistry. 272 (38): 24081–7. doi:10.1074/jbc.272.38.24081. PMID 9295362.

Further reading

• Chen Y, Sharp ZD, Lee WH (Sep 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". The Journal of Biological Chemistry. 272 (38): 24081–7. doi:10.1074/jbc.272.38.24081. PMID 9295362.
• Zheng L, Chen Y, Lee WH (Aug 1999). "Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins". Molecular and Cellular Biology. 19 (8): 5417–28. doi:10.1128/mcb.19.8.5417. PMC 84384. PMID 10409732.
• Zheng L, Chen Y, Riley DJ, Chen PL, Lee WH (May 2000). "Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p". Molecular and Cellular Biology. 20 (10): 3529–37. doi:10.1128/MCB.20.10.3529-3537.2000. PMC 85645. PMID 10779342.
• Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH (Dec 2002). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". The Journal of Biological Chemistry. 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167.
• Tien AC, Lin MH, Su LJ, Hong YR, Cheng TS, Lee YC, Lin WJ, Still IH, Huang CY (Jan 2004). "Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model". Molecular & Cellular Proteomics. 3 (1): 93–104. doi:10.1074/mcp.M300072-MCP200. PMID 14602875.
• DeLuca JG, Howell BJ, Canman JC, Hickey JM, Fang G, Salmon ED (Dec 2003). "Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores". Current Biology. 13 (23): 2103–9. doi:10.1016/j.cub.2003.10.056. PMID 14654001.
• Bharadwaj R, Qi W, Yu H (Mar 2004). "Identification of two novel components of the human NDC80 kinetochore complex". The Journal of Biological Chemistry. 279 (13): 13076–85. doi:10.1074/jbc.M310224200. PMID 14699129.
• Lou Y, Yao J, Zereshki A, Dou Z, Ahmed K, Wang H, Hu J, Wang Y, Yao X (May 2004). "NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling". The Journal of Biological Chemistry. 279 (19): 20049–57. doi:10.1074/jbc.M314205200. PMID 14978040.
• Joseph J, Liu ST, Jablonski SA, Yen TJ, Dasso M (Apr 2004). "The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo". Current Biology. 14 (7): 611–7. doi:10.1016/j.cub.2004.03.031. PMID 15062103.
• Steensgaard P, Garrè M, Muradore I, Transidico P, Nigg EA, Kitagawa K, Earnshaw WC, Faretta M, Musacchio A (Jun 2004). "Sgt1 is required for human kinetochore assembly". EMBO Reports. 5 (6): 626–31. doi:10.1038/sj.embor.7400154. PMC 1299074. PMID 15133482.
• Stucke VM, Baumann C, Nigg EA (Aug 2004). "Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1". Chromosoma. 113 (1): 1–15. doi:10.1007/s00412-004-0288-2. PMID 15235793.
• Meraldi P, Draviam VM, Sorger PK (Jul 2004). "Timing and checkpoints in the regulation of mitotic progression". Developmental Cell. 7 (1): 45–60. doi:10.1016/j.devcel.2004.06.006. PMID 15239953.
• Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A (Sep 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes & Development. 18 (18): 2255–68. doi:10.1101/gad.1234104. PMC 517519. PMID 15371340.
• Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology. 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821.
• Le XF, Lammayot A, Gold D, Lu Y, Mao W, Chang T, Patel A, Mills GB, Bast RC (Jan 2005). "Genes affecting the cell cycle, growth, maintenance, and drug sensitivity are preferentially regulated by anti-HER2 antibody through phosphatidylinositol 3-kinase-AKT signaling". The Journal of Biological Chemistry. 280 (3): 2092–104. doi:10.1074/jbc.M403080200. PMID 15504738.
• DeLuca JG, Dong Y, Hergert P, Strauss J, Hickey JM, Salmon ED, McEwen BF (Feb 2005). "Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites". Molecular Biology of the Cell. 16 (2): 519–31. doi:10.1091/mbc.E04-09-0852. PMC 545888. PMID 15548592.