16S rRNA (cytosine967-C5)-methyltransferase

16S rRNA (cytosine⁹⁶⁷-C⁵)-methyltransferase (EC 2.1.1.176, rsmB (gene), fmu (gene), 16S rRNA m5C967 methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:16S rRNA (cytosine⁹⁶⁷-C⁵)-methyltransferase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + cytosine⁹⁶⁷ in 16S rRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + 5-methylcytosine⁹⁶⁷ in 16S rRNA

The enzyme specifically methylates cytosine⁹⁶⁷ at C⁵ in 16S rRNA.

References

↑ Tscherne, J.S.; Nurse, K.; Popienick, P.; Michel, H.; Sochacki, M.; Ofengand, J. (1999). "Purification, cloning, and characterization of the 16S RNA m⁵C⁹⁶⁷ methyltransferase from Escherichia coli". Biochemistry. 38: 1884–1892. doi:10.1021/bi981880l. PMID 10026269.
↑ Gu, X.R.; Gustafsson, C.; Ku, J.; Yu, M.; Santi, D.V. (1999). "Identification of the 16S rRNA m⁵C⁹⁶⁷ methyltransferase from Escherichia coli". Biochemistry. 38: 4053–4057. doi:10.1021/bi982364y. PMID 10194318.
↑ Foster, P.G.; Nunes, C.R.; Greene, P.; Moustakas, D.; Stroud, R.M. (2003). "The first structure of an RNA m⁵C methyltransferase, Fmu, provides insight into catalytic mechanism and specific binding of RNA substrate". Structure. 11: 1609–1620. doi:10.1016/j.str.2003.10.014. PMID 14656444.

External links

16S rRNA (cytosine967-C5)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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