23S rRNA (uracil1939-C5)-methyltransferase

23S rRNA (uracil^1939-C^5)-methyltransferase
Identifiers
EC number	2.1.1.190
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

23S rRNA (uracil¹⁹³⁹-C⁵)-methyltransferase (EC 2.1.1.190, RumA, RNA uridine methyltransferase A, YgcA) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (uracil¹⁹³⁹-C⁵)-methyltransferase.^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + uracil¹⁹³⁹ in 23S rRNA $\rightleftharpoons$ S-adenosyl-L-homocysteine + 5-methyluracil¹⁹³⁹ in 23S rRNA

The enzyme specifically methylates uracil¹⁹³⁹ at C⁵ in 23S rRNA.

References

1. Agarwalla, S.; Kealey, J.T.; Santi, D.V.; Stroud, R.M. (2002). "Characterization of the 23 S ribosomal RNA m⁵U¹⁹³⁹ methyltransferase from Escherichia coli". J. Biol. Chem. 277 (11): 8835–8840. doi:10.1074/jbc.M111825200. PMID 11779873.
2. Lee, T.T.; Agarwalla, S.; Stroud, R.M. (2004). "Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase". Structure. 12 (3): 397–407. doi:10.1016/j.str.2004.02.009. PMID 15016356.
3. Madsen, C.T.; Mengel-Jorgensen, J.; Kirpekar, F.; Douthwaite, S. (2003). "Identifying the methyltransferases for m⁵U⁷⁴⁷ and m⁵U¹⁹³⁹ in 23S rRNA using MALDI mass spectrometry". Nucleic Acids Res. 31: 4738–4746. doi:10.1093/nar/gkg657. PMID 12907714.
4. Persaud, C.; Lu, Y.; Vila-Sanjurjo, A.; Campbell, J.L.; Finley, J.; O'Connor, M. (2010). "Mutagenesis of the modified bases, m⁵U¹⁹³⁹ and Ψ²⁵⁰⁴, in Escherichia coli 23S rRNA". Biochem. Biophys. Res. Commun. 392 (2): 223–227. doi:10.1016/j.bbrc.2010.01.021. PMID 20067766.
5. Agarwalla, S.; Stroud, R.M.; Gaffney, B.J. (2004). "Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies". J. Biol. Chem. 279 (33): 34123–34129. doi:10.1074/jbc.M405702200. PMID 15181002.
6. Lee, T.T.; Agarwalla, S.; Stroud, R.M. (2005). "A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function". Cell. 120 (5): 599–611. doi:10.1016/j.cell.2004.12.037. PMID 15766524.

External links

• 23S rRNA (uracil1939-C5)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)