23S rRNA (uridine2552-2'-O)-methyltransferase

23S rRNA (uridine²⁵⁵2-2'-O)-methyltransferase (EC 2.1.1.166, Um(2552) 23S ribosomal RNA methyltransferase, heat shock protein RrmJ, RrmJ, FTSJ, Um²⁵⁵² methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (uridine²⁵⁵²-2'-O-)-methyltransferase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + uridine²⁵⁵² in 23S rRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + 2'-O-methyluridine²⁵⁵² in 23S rRNA

The enzyme catalyses the 2'-O-methylation of the universally conserved U²⁵⁵² in the A loop of 23S rRNA.

References

↑ Caldas, T.; Binet, E.; Bouloc, P.; Costa, A.; Desgres, J.; Richarme, G. (2000). "The FtsJ/RrmJ heat shock protein of Escherichia coli is a 23 S ribosomal RNA methyltransferase". J. Biol. Chem. 275 (22): 16414–16419. doi:10.1074/jbc.M001854200. PMID 10748051.
↑ Hager, J.; Staker, B.L.; Bugl, H.; Jakob, U. (2002). "Active site in RrmJ, a heat shock-induced methyltransferase". J. Biol. Chem. 277 (44): 41978–41986. doi:10.1074/jbc.M205423200. PMID 12181314.
↑ Hager, J.; Staker, B.L.; Jakob, U. (2004). "Substrate binding analysis of the 23S rRNA methyltransferase RrmJ". J. Bacteriol. 186 (19): 6634–6642. doi:10.1128/JB.186.19.6634-6642.2004. PMC 516604. PMID 15375145.
↑ Bugl, H.; Fauman, E.B.; Staker, B.L.; Zheng, F.; Kushner, S.R.; Saper, M.A.; Bardwell, J.C.; Jakob, U. (2000). "RNA methylation under heat shock control". Mol. Cell. 6 (2): 349–360. doi:10.1016/S1097-2765(00)00035-6. PMID 10983982.

External links

23S rRNA (uridine2552-2'-O)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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