Binding domain

A binding domain is a protein domain which binds to a specific atom or molecule, such as calcium or DNA. Upon binding, proteins may undergo a conformational change. Binding domains are essential for the function of many proteins.

Examples of binding domains include Zinc finger, which binds to DNA, and EF hand, which binds to calcium.

Proteins: key methods of study

Experimental	Protein purification Green fluorescent protein Western blot Protein immunostaining Protein sequencing Gel electrophoresis/Protein electrophoresis Protein immunoprecipitation Peptide mass fingerprinting/Protein mass spectrometry Dual-polarization interferometry Microscale thermophoresis Chromatin immunoprecipitation Surface plasmon resonance Isothermal titration calorimetry X-ray crystallography Protein NMR Cryo-electron microscopy Freeze-fracture electron microscopy

Bioinformatics	Protein structure prediction Protein–protein docking Protein structural alignment Protein ontology Protein–protein interaction prediction

Assay	Enzyme assay Protein assay Secretion assay

Display techniques	Bacterial display mRNA display Phage display Ribosome display Yeast display

Super-resolution microscopy	Photoactivated localization microscopy Vertico SMI

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Binding domain

See also