Moraxella catarrhalis

Moraxella catarrhalis
Scientific classification
Kingdom: Bacteria
Phylum: Proteobacteria
Class: Gammaproteobacteria
Order: Pseudomonadales
Family: Moraxellaceae
Genus: Moraxella
Species: M. catarrhalis
Binomial name
Moraxella catarrhalis

Moraxella catarrhalis is a fastidious, nonmotile, Gram-negative, aerobic, oxidase-positive diplococcus that can cause infections of the respiratory system, middle ear, eye, central nervous system, and joints of humans. It causes the infection of the host cell by sticking to the host cell using trimeric autotransporter adhesins.

Epidemiology

M. catarrhalis is a human pathogen with an affinity for the human upper respiratory tract. Other primates, such as macaques, might become infected by this bacterium.[1]

History

M. catarrhalis was previously placed in a separate genus named Branhamella. The rationale for this was that other members of the genus Moraxella are rod-shaped and rarely caused infections in humans. However, results from DNA hybridization studies and 16S rRNA sequence comparisons were used to justify inclusion of the species M. catarrhalis in the genus Moraxella. As a consequence, the name Moraxella catarrhalis is currently preferred for these bacteria. Nevertheless, some in the medical field continue to call these bacteria Branhamella catarrhalis.

Moraxella is named after Victor Morax, a Swiss ophthalmologist who first described this genus of bacteria. Catarrhalis is derived from catarrh, from the Greek meaning "to flow down" (cata- implies down; -rrh implies flow), describing the profuse discharge from eyes and nose typically associated with severe inflammation in colds.

Genetics

The whole genome sequence of M. catarrhalis CCUG 353 type strain was deposited and published in DNA Data Bank of Japan, European Nucleotide Archive, and GenBank in 2016 under the accession number LWAH00000000.[2]

Clinical significance

These bacteria are known to cause otitis media,[3][4] bronchitis, sinusitis, and laryngitis. Elderly patients and long-term heavy smokers with chronic pulmonary disease should be aware that M. catarrhalis is associated with bronchopneumonia, as well as exacerbations of existing chronic obstructive pulmonary disease.

The peak rate of colonisation by M. catarrhalis appears to occur around 2 years of age, with a striking difference in colonization rates between children and adults (very high to very low).

M. catarrhalis has recently been gaining attention as an emerging human pathogen. It has been identified as an important cause in bronchopulmonary infection, causing infection through pulmonary aspiration in the upper pulmonary tract.[5] Additionally, it causes bacterial pneumonia, especially in adults with a compromised immune system.[6] It has also been known to cause infective exacerbations in adults with chronic lung disease, and it is an important cause in acute sinusitis, maxillary sinusitis, bacteremia, meningitis, conjunctivitis, acute purulent irritation of chronic bronchitis, urethritis, septicemia (although this is rare), septic arthritis (which is also a rare occurrence),and acute laryngitis in adults and acute otitis media in children.[7][8] M. catarrhalis is an opportunistic pulmonary invader, and causes harm especially in patients who have compromised immune systems or any underlying chronic disease.[5][7]

M. catarrhalis has also been linked with septic arthritis in conjunction with bacteremia.[7] Although cases of bacteremia cased by M. catarrhalis have been reported before, this was the first instance in which bacteremia caused by M. catarrhalis was also associated with septic arthritis. A microbiological evaluation of the patient (a 41-year-old male) revealed that M. catarrhalis was the cause of the disease rather than Neisseria as was previously believed. This was also the second case of M. catarrhalis causing septic arthritis (although in the first case, no mention of bacteremia was made).[7]

Along with its relation to septic arthritis, bacteremia is also caused by M. catarrhalis infection, which can range in severity from a slight fever to lethal sepsis and an associated respiratory tract infection is usually also identified.[9] Bacteremia infections caused by M. catarrhalis have a 21% mortality rate among patients. However, this may have been due to a lack of knowledge about the bacterium because of its recent recognition as a pathogen.[9]

Infection of high-grade bacteremia was linked with the development of endocarditis.[9] However, the patients without endocarditis has been related to the background of each patient, especially the existence of other illnesses and any possible immune impairments they may have. Also, although bacteremia caused by M. catarrhalis has been infrequently reported, this may be due to a misdiagnosis or oversight because M. catarrhalis was only recently (1990s) identified as an important pathogen.[9] Many chronic diseases in patients with M. catarrhalis bacteremia can be linked to the patients with immune defects or respiratory debility. Likewise, respiratory debility in patients with bacteremic pneumonia caused by M. catarrhalis infection can be linked with increased rates of pharyngeal colonization, enhancement of bacterial adherence to abnormal epithelium, and increased susceptibility of pulmonary parenchyma to infection.[9]

Antibiotic resistance

Antibiotic sensitivity test: This strain shows resistance to ampicillin because it produces the enzyme β-lactamase. This is confirmed by the disc labelled β turning red.

M. catarrhalis can be treated with antibiotics, but it is commonly resistant to penicillin, ampicillin, and amoxicillin.[9]

Current research priorities involve trying to find a suitable vaccine[10] for this genotypically diverse organism, as well as determining factors involved with virulence, e.g. complement resistance. Lipooligosaccharide is considered one possible virulence factor.[10]

Since the recent recognition of M. catarrhalis as an important pathogenic microbe, development of a possible antibiotic has been ongoing. A fraction of M. catarrhalis strains seemed to be resistant to ampicillin, which makes ampicillin and amoxicillin inappropriate choices of antibiotic against it.[5] Although all strains of M. catarrhalis were susceptible to cotrimoxazole, erythromycin, sulfadimidine, and tetracycline, they were also resistant to trimethoprim.[5] One of the reasons for the resistance of M. catarrhalis to ampicillin and trimethoprim is because of the beta-lactamase production, which allows resistance to ampicillin.[5] Also, the resistance of M. catarrhalis to other antibiotics may be attributed to beta-lactamase, as well, because the use of these antibiotics has triggered an increase in development of beta-lactamase, which resists antibiotics.[5]

However, a 1994 study has identified a large protein on the surface of M. catarrhalis that may serve as a target for protective antibodies.[6] This UspA (the designated antigen) protein is the first surface-exposed protein on M. catarrhalis that can be a target for biologically active antibodies, and therefore lead to a vaccination. This protein was also present in all of the strains tested. The large size of the exposed protein macromolecule makes it similar to Neisseria gonorrhoeae outer membrane protein macromolecular complex, which implies that UspA may be a single polypeptide chain.[6]

Active immunization, in a study, of M. catarrhalis in the respiratory tract allowed the control of the growth of M. catarrhalis and led to the development of serum antigens.[8] Also, an enhanced ability exists in the test subjects (mice) to clear M. catarrhalis from their lungs. Likewise, passive immunization of M. catarrhalis from the mice respiratory tracts also enhanced the mice’s ability to clear the microbes from their lungs, which means that serum antibodies likely play a large role in the immunization and protection of the respiratory tract.[8] Along with outer membrane proteins that are consistent among different strains of M. catarrhalis, a sort of subclass-specific IgG antibody response to certain outer membrane proteins may also exist. Therefore, the outer membrane antigens of M. catarrhalis also provide a possible vaccine source. Also, a bactericidal serum antibody has also been developed in response to the diseases caused by M. catarrhalis.[8]

Treatment

Treatment options include antibiotic therapy or a so-called "watchful waiting" approach. The great majority of clinical isolates of this organism produce beta-lactamases, so are resistant to penicillin. Resistance to trimethoprim, trimethoprim-sulfamethoxazole (TMP-SMX), clindamycin, and tetracycline have been reported. It is susceptible to fluoroquinolones, most second- and third-generation cephalosporins, erythromycin, and amoxicillin-clavulanate.

Vaccine development

Currently, no vaccine is known in the US against M. catarrhalis infection. It is a significant cause of respiratory tract infections against which a vaccine is sought. Several outer membrane proteins are currently under investigation as potential vaccine antigens, including the porin M35.

Biochemistry

During the first reported case of M. catarrhalis causing bacteremia that was associated with septic arthritis, the microbe was cultured, which revealed much about the morphology of its colonies, as well as M. catarrhalis itself.[7] M. catarrhalis is a large, kidney-shaped, Gram-negative diplococcus. It can be cultured on blood and chocolate agar plates after an aerobic incubation at 37°C for 24 hours. Cultures revealed gray-white hemispheric colonies about 1 mm in diameter. These colonies were fragile and easy to crumble, and appeared to have a waxy surface.[7]

The hockey puck test was applied to these M. catarrhalis colonies,[7] in which a wooden stick is used to try to push the colonies across the plate. The M. catarrhalis colonies scored positively on this test, which means they could be slid across the plate. The colonies did not demonstrate hemolysis, and were not able to ferment glucose, sucrose, maltose, or lactose. They were able to produce DNase. Cultures of the M. catarrhalis tested positive for oxidase and nitrate reduction, which is characteristic of M. catarrhalis.[7] Many laboratories also perform a butyrate esterase test and a beta-lactamase test. Both tests should be positive and can help to rapidly identify it from a culture.[11]

The recognition of M. catarrhalis as a pathogen has led to studies for possible antibodies against it, which have led to a wider understanding of theits composition. The outer membrane protein (OMP) profiles of different strains of M. catarrhalis are extremely similar to each other.[6] Analyses of these OMP profiles with monoclonal antibodies (MAbs) revealed that a few proteins with similar molecular masses in the different strains have cross-reactive epitopes.[6] Also, a surface-exposed protein on M. catarrhalis has an unusually high molecular mass. An 80-kDa OMP on M. catarrhalis is immunogenic and common to all nonencapsulated strands of M. catarrhalis, which suggests it may be used as an antigen for immunization.[6]

References

  1. ↑ Embers, Monica E.; Doyle LA; Whitehouse CA; Selby EB; Chappell M; Philipp MT (27 January 2011). "Characterization of a Moraxella species that causes epistaxis in macaques". Veterinary Microbiology. 147 (3-4): 367–375. doi:10.1016/j.vetmic.2010.06.029. PMID 20667430. Retrieved 2 October 2013.
  2. ↑ Jakobsson, Hedvig E.; Salvà-Serra, Francisco; Thorell, Kaisa; Gonzales-Siles, Lucia; Boulund, Fredrik; Karlsson, Roger; Sikora, Per; Engstrand, Lars; Kristiansson, Erik (2016-06-30). "Draft Genome Sequence of Moraxella catarrhalis Type Strain CCUG 353T". Genome Announcements. 4 (3): e00552–16. doi:10.1128/genomeA.00552-16. ISSN 2169-8287. PMC 4911475Freely accessible. PMID 27313296.
  3. ↑ Mawas F, Ho MM, Corbel MJ (January 2009). "Current progress with Moraxella catarrhalis antigens as vaccine candidates". Expert Rev Vaccines. 8 (1): 77–90. doi:10.1586/14760584.8.1.77. PMID 19093775.
  4. ↑ Yu S, Gu XX (June 2007). "Biological and Immunological Characteristics of Lipooligosaccharide-Based Conjugate Vaccines for Serotype C Moraxella catarrhalis". Infect. Immun. 75 (6): 2974–80. doi:10.1128/IAI.01915-06. PMC 1932890Freely accessible. PMID 17371852.
  5. 1 2 3 4 5 6 Winstanley, T.G. and R.C. Spencer. “Moraxea catarrkar: antibiotic susceptibility with special reference to trimethoprim.” Journal of Antimicrobial Chemotherapy 18, no. 3 (1986): 425-426.
  6. 1 2 3 4 5 6 Helminen, Merja E., Isobel Maciver, Jo L. Latimer, Julia Klesney-Tait, Leslie D. Cope, Maria Paris, George H. McCracken, Jr., and Eric J. Hansen. “A Large, Antigenically Conserved Protein on the Surface of Moraxella catarrhalis Is a Target for Protective Antibodies.” The Journal of Infectious Diseases 170, no. 4 (1994): 867-872.
  7. 1 2 3 4 5 6 7 8 Melendez, Philipp R. and Royce H. Johnson. “Bacteremia and Septic Arthritis Caused by Moraxella catarrhalis.” Reviews of Infectious Diseases 13, no. 3 (1991): 428-429.
  8. 1 2 3 4 Maciver, Isobel, Manaswat Unhanand, George H. McCracken Jr., and Eric J. Hansen. “Effect of Immunization on Pulmonary Clearance of Moraxella Catarrhalis in an Animal Model.” The Journal of Infectious Diseases 168, no. 2 (1993): 469-472.
  9. 1 2 3 4 5 6 Ioannidis, John P. A. Ioannidis, Michael Worthington, Jeffrey K. Griffiths, and David R. Snydman. "Spectrum and Significance of Bacteremia Due to Moraxella catarrhalis." Clinical Infectious Diseases 21, no. 2 (1995): 390-397.
  10. 1 2 Peng D, Hong W, Choudhury BP, Carlson RW, Gu XX (November 2005). "Moraxella catarrhalis Bacterium without Endotoxin, a Potential Vaccine Candidate". Infect. Immun. 73 (11): 7569–77. doi:10.1128/IAI.73.11.7569-7577.2005. PMC 1273912Freely accessible. PMID 16239560.
  11. ↑ Manual of Clinical Microbiology, 10th edition. James Versalovic, copyright 2012 ASM press.
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