mRNA (nucleoside-2'-O-)-methyltransferase

Thus, the two substrates of this enzyme are S-adenosyl methionine and m7G(5')pppR-RNA, whereas its two products are S-adenosylhomocysteine and m7G(5')pppRm-RNA (mRNA containing a 2'-O-methylpurine cap).

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:mRNA (nucleoside-2'-O-)-methyltransferase. Other names in common use include messenger ribonucleate nucleoside 2'-methyltransferase, and messenger RNA (nucleoside-2'-)-methyltransferase.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2GA9 and 2GAF.

References

Barbosa E, Moss B (1978). "mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus Purification and physical properties". J. Biol. Chem. 253 (21): 7692–7. PMID 701281.
Barbosa E, Moss B (1978). "mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus Characteristics and substrate specificity". J. Biol. Chem. 253 (21): 7698–702. PMID 701282.
Boone RF, Ensinger MJ, Moss B (1977). "Synthesis of mRNA guanylyltransferase and mRNA methyltransferases in cells infected with vaccinia virus". J. Virol. 21 (2): 475–83. PMC 353847. PMID 833934.
Ensinger MJ, Martin SA, Paoletti E, Moss B (1975). "Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus". Proc. Natl. Acad. Sci. U.S.A. 72 (7): 2525–9. doi:10.1073/pnas.72.7.2525. PMC 432801. PMID 1058472.
Groner Y, Gilboa E, Aviv H (1978). "Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates". Biochemistry. 17 (6): 977–82. doi:10.1021/bi00599a005. PMID 629955.

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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