DDX20

DDX20
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2OXC, 3B7G
Identifiers
Aliases	DDX20, DP103, GEMIN3, DEAD-box helicase 20
External IDs	MGI: 1858415 HomoloGene: 5214 GeneCards: DDX20
Gene ontology Molecular function • DNA binding • protein binding, bridging • nucleotide binding • ATP-dependent RNA helicase activity • helicase activity • protein domain specific binding • histone deacetylase binding • protein binding • nucleic acid binding • repressing transcription factor binding • hydrolase activity • ATP binding Cellular component • cytoplasm • SMN-Sm protein complex • cytosol • membrane • transcriptional repressor complex • nucleoplasm • SMN complex • RNA polymerase II transcription repressor complex • cytoskeleton • nucleus • Gemini of coiled bodies Biological process • RNA processing • spliceosomal tri-snRNP complex assembly • mRNA processing • regulation of steroid biosynthetic process • negative regulation of transcription from RNA polymerase II promoter • oogenesis • spliceosomal snRNP assembly • RNA secondary structure unwinding • RNA splicing • positive regulation of apoptotic process • negative regulation of transcription, DNA-templated • negative regulation of cell proliferation • nuclear import Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	11218	53975
Ensembl	ENSG00000064703	ENSMUSG00000027905
UniProt	Q9UHI6	Q9JJY4
RefSeq (mRNA)	NM_007204	NM_017397
RefSeq (protein)	NP_009135.4	NP_059093.3
Location (UCSC)	Chr 1: 111.76 – 111.77 Mb	Chr 3: 105.68 – 105.69 Mb
PubMed search	[1]	[2]
Wikidata

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Probable ATP-dependent RNA helicase DDX20, also known as DEAD-box helicase 20 and gem-associated protein 3 (GEMIN3), is an enzyme that in humans is encoded by the DDX20 gene.^[3][4]

Function

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neuron (SMN) complex.^[4] This DEAD box protein is one of the gem-associated proteins (GEMIN3) that interacts directly with SMN.^[5] SMN is the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.^[4]

Interactions

DDX20 has been shown to interact with:

• EIF2C2,^[6][7]
• GEMIN4,^[6][8][9]
• GEMIN5,^[6][10]
• LSM2,^[11]
• SMN1,^{[6][8][11][12]}
• SNRPB,^[11]
• SNRPD3^[11] and
• SNRPG,^[11]
• SNRPD1,^[11]
• SNRPD2,^[11]
• SNRPE,^[11]
• SNRPF,^[11] and
• SIP1.^[6][11]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Grundhoff AT, Kremmer E, Türeci O, Glieden A, Gindorf C, Atz J, Mueller-Lantzsch N, Schubach WH, Grässer FA (July 1999). "Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C". J Biol Chem. 274 (27): 19136–44. doi:10.1074/jbc.274.27.19136. PMID 10383418.
4. "Entrez Gene: DDX20 DEAD (Asp-Glu-Ala-Asp) box polypeptide 20".
5. Seng, CO; Magee, C; Young, PJ; Lorson, CL; Allen, JP (15 April 2015). "The SMN structure reveals its crucial role in snRNP assembly.". Human Molecular Genetics. 24 (8): 2138–46. doi:10.1093/hmg/ddu734. PMID 25561692.
6. Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rappsilber J, Mann M, Dreyfuss G (March 2002). "miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs". Genes Dev. 16 (6): 720–8. doi:10.1101/gad.974702. PMC 155365. PMID 11914277.
7. Nelson PT, Hatzigeorgiou AG, Mourelatos Z (March 2004). "miRNP:mRNA association in polyribosomes in a human neuronal cell line". RNA. 10 (3): 387–94. doi:10.1261/rna.5181104. PMC 1370934. PMID 14970384.
8. Carnegie GK, Sleeman JE, Morrice N, Hastie CJ, Peggie MW, Philp A, Lamond AI, Cohen PT (May 2003). "Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs". J. Cell. Sci. 116 (Pt 10): 1905–13. doi:10.1242/jcs.00409. PMID 12668731.
9. Charroux B, Pellizzoni L, Perkinson RA, Yong J, Shevchenko A, Mann M, Dreyfuss G (March 2000). "Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli". J. Cell Biol. 148 (6): 1177–86. doi:10.1083/jcb.148.6.1177. PMC 2174312. PMID 10725331.
10. Gubitz AK, Mourelatos Z, Abel L, Rappsilber J, Mann M, Dreyfuss G (February 2002). "Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins". J. Biol. Chem. 277 (7): 5631–6. doi:10.1074/jbc.M109448200. PMID 11714716.
11. Charroux B, Pellizzoni L, Perkinson RA, Shevchenko A, Mann M, Dreyfuss G (December 1999). "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems". J. Cell Biol. 147 (6): 1181–94. doi:10.1083/jcb.147.6.1181. PMC 2168095. PMID 10601333.
12. Meister G, Bühler D, Laggerbauer B, Zobawa M, Lottspeich F, Fischer U (August 2000). "Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins". Hum. Mol. Genet. 9 (13): 1977–86. doi:10.1093/hmg/9.13.1977. PMID 10942426.

Further reading

• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library construction.". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (1997). "Large-scale concatenation cDNA sequencing.". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
• Charroux B, Pellizzoni L, Perkinson RA, Shevchenko A, Mann M, Dreyfuss G (2000). "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems.". J. Cell Biol. 147 (6): 1181–94. doi:10.1083/jcb.147.6.1181. PMC 2168095. PMID 10601333.
• Charroux B, Pellizzoni L, Perkinson RA, Yong J, Shevchenko A, Mann M, Dreyfuss G (2000). "Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli.". J. Cell Biol. 148 (6): 1177–86. doi:10.1083/jcb.148.6.1177. PMC 2174312. PMID 10725331.
• Meister G, Bühler D, Laggerbauer B, Zobawa M, Lottspeich F, Fischer U (2000). "Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins.". Hum. Mol. Genet. 9 (13): 1977–86. doi:10.1093/hmg/9.13.1977. PMID 10942426.
• Pellizzoni L, Charroux B, Rappsilber J, Mann M, Dreyfuss G (2001). "A functional interaction between the survival motor neuron complex and RNA polymerase II.". J. Cell Biol. 152 (1): 75–85. doi:10.1083/jcb.152.1.75. PMC 2193649. PMID 11149922.
• Friesen WJ, Paushkin S, Wyce A, Massenet S, Pesiridis GS, Van Duyne G, Rappsilber J, Mann M, Dreyfuss G (2001). "The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins.". Mol. Cell. Biol. 21 (24): 8289–300. doi:10.1128/MCB.21.24.8289-8300.2001. PMC 99994. PMID 11713266.
• Gubitz AK, Mourelatos Z, Abel L, Rappsilber J, Mann M, Dreyfuss G (2002). "Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins.". J. Biol. Chem. 277 (7): 5631–6. doi:10.1074/jbc.M109448200. PMID 11714716.
• Pellizzoni L, Baccon J, Rappsilber J, Mann M, Dreyfuss G (2002). "Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component.". J. Biol. Chem. 277 (9): 7540–5. doi:10.1074/jbc.M110141200. PMID 11748230.
• Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rappsilber J, Mann M, Dreyfuss G (2002). "miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.". Genes Dev. 16 (6): 720–8. doi:10.1101/gad.974702. PMC 155365. PMID 11914277.
• Klappacher GW, Lunyak VV, Sykes DB, Sawka-Verhelle D, Sage J, Brard G, Ngo SD, Gangadharan D, Jacks T, Kamps MP, Rose DW, Rosenfeld MG, Glass CK (2002). "An induced Ets repressor complex regulates growth arrest during terminal macrophage differentiation.". Cell. 109 (2): 169–80. doi:10.1016/S0092-8674(02)00714-6. PMID 12007404.
• Baccon J, Pellizzoni L, Rappsilber J, Mann M, Dreyfuss G (2002). "Identification and characterization of Gemin7, a novel component of the survival of motor neuron complex.". J. Biol. Chem. 277 (35): 31957–62. doi:10.1074/jbc.M203478200. PMID 12065586.
• Narayanan U, Ospina JK, Frey MR, Hebert MD, Matera AG (2003). "SMN, the spinal muscular atrophy protein, forms a pre-import snRNP complex with snurportin1 and importin beta.". Hum. Mol. Genet. 11 (15): 1785–95. doi:10.1093/hmg/11.15.1785. PMC 1630493. PMID 12095920.
• Carnegie GK, Sleeman JE, Morrice N, Hastie CJ, Peggie MW, Philp A, Lamond AI, Cohen PT (2004). "Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs.". J. Cell. Sci. 116 (Pt 10): 1905–13. doi:10.1242/jcs.00409. PMID 12668731.
• Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region.". Genomics. 83 (1): 153–67. doi:10.1016/S0888-7543(03)00235-0. PMID 14667819.
• Nelson PT, Hatzigeorgiou AG, Mourelatos Z (2004). "miRNP:mRNA association in polyribosomes in a human neuronal cell line.". RNA. 10 (3): 387–94. doi:10.1261/rna.5181104. PMC 1370934. PMID 14970384.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins.". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.