Lung microbiome

The lung microbiota (or pulmonary microbial community) is a complex variety of microbes found in the lower respiratory tract particularly on the mucus layer and the epithelial surfaces (the lung microbiome refer to their genomes). These lung microbes include bacteria, fungi, viruses and bacteriophages. The bacterial part of microbiome has been addressed more deeply. It consists of 9 core genera: Prevotella, Sphingomonas, Pseudomonas, Acinetobacter, Fusobacterium, Megasphaera, Veillonella, Staphylococcus, and Streptococcus.[1][2][3] They are aerobes as well as anaerobes and aerotolerant bacteria. The microbial communities are highly variable in particular individuals and compose of about 140 distinct families. The bronchial tree for instance contains a mean of 2000 bacterial genomes per cm2 surface. The harmful or potentially harmful bacteria are also detected routinely in respiratory specimens. The most significant are Moraxella catarrhalis, Haemophilus influenzae, and Streptococcus pneumoniae. They are known to cause respiratory disorders under particular conditions namely if the human immune system is impaired. The mechanism by which they persist in the lower airways in healthy individuals is unknown.

Fungal genera that are commonly found in the pulmonary microbiome include Candida, Malassezia, Neosartorya, Saccharomyces, and Aspergillus, among others.[4]

Role of the epithelial barrier

The airway epithelium together with alveolar macrophages and dendritic cells play a major role in the initial recognition of bacterial products getting into the lower airways with the air. Since some of these products are potent proinflammatory stimuli it is extremely important for the immune system to distinguish between pathogens and non-pathogenic commensals. This prevents the development of constant inflammation and forms tolerance against harmless microbiota.[5]

Mechanisms underlying inflammation. The airway epithelium has a complex structure consisting of at least seven diverse cell types interacting with each other by means of tight junctions. Epithelial cells can transmit immunostimulatory signals to underlying tissues taking part in the mechanisms of innate and adaptive immune response. The key transmitters of these signals are dendritic cells. Once pathogenic bacteria (e.g., S. pneumoniae, P. aeruginosa) have activated particular pattern recognition receptors on/in epithelial cells, the proinflammatory signaling pathways are activated. This results mainly in IL-1, IL-6 and IL-8 production. These cytokines induce chemotaxis to the site of infection in its target cells (e.g., neutrophils, dendritic cells and macrophages). On the other hand, representatives of standard microbiota induce only weak signals preventing inflammation. The mechanism of distinguishing between harmless and harmful bacteria on the molecular as well as on physiological levels is not completely understood.

This process becomes much more intriguing when taking into account that commensals often share their surface molecules with pathogens. Epithelial cells are equipped with very sensitive recognition tools - toll like receptors (TLRs), nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) and retinoic acid-inducible gene (RIG)-I-like receptors (RLRs) which recognize a broad variety of microbial structural components. After recognition of pathogenic bacteria proinflammatory pathways are activated and cellular components of the adaptive and innate immunity are recruited to the infection site.[6] One key regulator in this process is the NF-κB which translocates from the cytoplasm into the nucleus and activates pro-inflammatory genes in epithelial cells and macrophages. The DNA-binding protein complex recognizes a discrete nucleotide sequence (5’-GGG ACT TTC T-3’) in the upstream region of a variety of response genes. The activation of NF-κB by a number of stimuli: bacterial cell walls or inflammatory cytokines results in its translocation to the nucleus.

In contrast, harmless bacteria do not cause the translocation of NF-κB into the nucleus thus preventing the inflammation although they can express the same microbe-associated molecular patterns (MAMPs). One possible mechanism explaining this effect was suggested by Neish showing that non-pathogenic S. typhimurium PhoPc and S. pullorum are able to prohibit the ubiquitination of NF-κB inhibitor molecule nuclear factor of NF-κB light polypeptide gene enhancer in B-cells inhibitor alpha (IκB-κ).[7] Another explanation of commensal tolerance of the epithelium refers to the post-translational modification of a protein by the covalent attachment of one or more ubiquitin (Ub) monomers. The inhibition of ubiquitination leads to reduction of inflammation, because only polyubiquitinated (IκB-κ is targeted for degradation by the 26 S proteasome, allowing NF-κB translocation to the nucleus and activation the transcription of effector genes (for example IL-8). Probiotic bacteria such as Lactobacilli are able to modulate the activity of the Ub-proteasome system via inducing reactive oxygen species (ROS) production in epithelial cells. In mammalian cells, ROS have been shown to serve as critical second messengers in multiple signal transduction pathways in response to proinflammatory cytokines. Bacterially induced ROS causes oxidative inactivation of the catalytic cysteine residue of Ub 12 resulting in incomplete but transient loss of cullin-1 neddylation and consequent effects on NF-κB and β-catenin signaling. Another commensal species, B. thetaiotaomicron, attenuates pro-inflammatory cytokine expression by promoting nuclear export of NF-κB subunit RelA, through a peroxisome proliferator activated receptor γ (PPAR-γ)-dependent pathway. PPAR-γ target transcriptionally active Rel A and induce early nuclear clearance limiting the duration of NF-κB action.

The balance between pathogens and commensals is extremely important in the maintenance of homeostasis in the respiratory tract.

Physiology

The airways are continually exposed to a multitude of microorganisms, some of which are able to persist and even colonize respiratory tract. This is possible due to the presence of nutrients, oxygen, and optimal growth temperature. There are several host-derived nutrient sources for microbial residents: secretions from airway epithelial cells (especially goblet cells), secretions from submucosal glands and transudate from plasma. Moreover, the pool of available nutrients is increased by the activities of some members of the microbiota. Macromolecular components of respiratory secretions (proteins, glycoproteins, lipids, nucleic acids) are converted to nutrients (e.g. carbohydrates, amino acids). Thus, the metabolic activity of present bacteria allow for the colonization of new species. The commensal bacteria are nonpathogenic and defend our airways against the pathogens. There are several possible mechanisms. Commensals are the native competitors of pathogenic bacteria, because they tend to occupy the same ecological niche inside the human body. Secondly, they are able to produce antibacterial substances called bacteriocins which inhibit the growth of pathogens.

Genera Bacillus, Lactobacillus, Lactococcus, Staphylococcus, Streptococcus, and Streptomyces are the main producers of bacteriocins in respiratory tract. Moreover, commensals are known to induce Th1 response and anti-inflammatory interleukin (IL)-10, antimicrobial peptides, FOXP3, secretory immunoglobulin A (sIgA) production.

Clinical significance

Changes in microbial community composition seem to play a role in progression of such pulmonary disorders as chronic obstructive pulmonary disease (COPD), asthma, and cystic fibrosis.[8][9] For example, presence of such genera as Mycoplasma, Pseudomonas, and Staphylococcus is correlated with stable COPD state. On the other hand, Prevotella, Mesorhizobium, Microbacterium, Micrococcus, Veillonela, Rhizobium, Stenotrophomonas, and Lactococcus present mostly in healthy individual cohort. The relative abundance of Proteobacteria is increased in asthmatic children. Pseudomonas aeruginosa, Staphylococcus aureus, and Burkholderia cepacia are found most often in cystic fibrosis patients.

High-throughput sequencing and the whole genome sequencing approaches will provide the further information about the complexity and physiological implication of commensal bacteria in the lower respiratory tract.

See also

References

  1. ↑ Erb-Downward, John R.; Thompson, Deborah L.; Han, Meilan K.; Freeman, Christine M.; McCloskey, Lisa; Schmidt, Lindsay A.; Young, Vincent B.; Toews, Galen B.; et al. (2011). Bereswill, Stefan, ed. "Analysis of the Lung Microbiome in the "Healthy" Smoker and in COPD". PLoS ONE. 6 (2): e16384. doi:10.1371/journal.pone.0016384. PMC 3043049Freely accessible. PMID 21364979.
  2. ↑ Hilty, Markus; Burke, Conor; Pedro, Helder; Cardenas, Paul; Bush, Andy; Bossley, Cara; Davies, Jane; Ervine, Aaron; et al. (2010). Neyrolles, Olivier, ed. "Disordered Microbial Communities in Asthmatic Airways". PLoS ONE. 5 (1): e8578. doi:10.1371/journal.pone.0008578. PMC 2798952Freely accessible. PMID 20052417.
  3. ↑ Beck, James M.; Young, Vincent B.; Huffnagle, Gary B. (1 February 2012). "The microbiome of the lung". Translational Research. 160 (4): 258–66. doi:10.1016/j.trsl.2012.02.005. PMC 3440512Freely accessible. PMID 22683412.
  4. ↑ Cui L, Morris A, Ghedin E (July 2013). "The human mycobiome in health and disease". Genome Med. 5 (7): 63. doi:10.1186/gm467. PMC 3978422Freely accessible. PMID 23899327. Figure 2: Distribution of fungal genera in different body sites
  5. ↑ Kumar, Himanshu; Kawai, Taro; Akira, Shizuo (2011). "Pathogen Recognition by the Innate Immune System". International Reviews of Immunology. 30 (1): 16–34. doi:10.3109/08830185.2010.529976. PMID 21235323.
  6. ↑ Sha, Q.; Truong-Tran, AQ; Plitt, JR; Beck, LA; Schleimer, RP (2004). "Activation of Airway Epithelial Cells by Toll-Like Receptor Agonists". American Journal of Respiratory Cell and Molecular Biology. 31 (3): 358–64. doi:10.1165/rcmb.2003-0388OC. PMID 15191912.
  7. ↑ Kumar, Amrita; Wu, Huixia; Collier-Hyams, Lauren S; Hansen, Jason M; Li, Tengguo; Yamoah, Kosj; Pan, Zhen-Qiang; Jones, Dean P; Neish, Andrew S (2007). "Commensal bacteria modulate cullin-dependent signaling via generation of reactive oxygen species". The EMBO Journal. 26 (21): 4457–66. doi:10.1038/sj.emboj.7601867. PMC 2063476Freely accessible. PMID 17914462.
  8. ↑ Huang, Yvonne J.; Kim, Eugenia; Cox, Michael J.; Brodie, Eoin L.; Brown, Ron; Wiener-Kronish, Jeanine P.; Lynch, Susan V. (2010). "A Persistent and Diverse Airway Microbiota Present during Chronic Obstructive Pulmonary Disease Exacerbations". OMICS: A Journal of Integrative Biology. 14: 9–59. doi:10.1089/omi.2009.0100.
  9. ↑ Cox, Michael J.; Allgaier, Martin; Taylor, Byron; Baek, Marshall S.; Huang, Yvonne J.; Daly, Rebecca A.; Karaoz, Ulas; Andersen, Gary L.; et al. (2010). Ratner, Adam J., ed. "Airway Microbiota and Pathogen Abundance in Age-Stratified Cystic Fibrosis Patients". PLoS ONE. 5 (6): e11044. doi:10.1371/journal.pone.0011044. PMC 2890402Freely accessible. PMID 20585638.
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