mRNA (nucleoside-2'-O-)-methyltransferase
mRNA (nucleoside-2'-O-)-methyltransferase | |||||||||
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Identifiers | |||||||||
EC number | 2.1.1.57 | ||||||||
CAS number | 61970-02-3 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
Gene Ontology | AmiGO / EGO | ||||||||
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In enzymology, a mRNA (nucleoside-2'-O-)-methyltransferase (EC 2.1.1.57) is an enzyme that catalyzes the chemical reaction
- S-adenosyl-L-methionine + m7G(5')pppR-RNA S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA (mRNA containing a 2'-O-methylpurine cap)
Thus, the two substrates of this enzyme are S-adenosyl methionine and m7G(5')pppR-RNA, whereas its two products are S-adenosylhomocysteine and m7G(5')pppRm-RNA (mRNA containing a 2'-O-methylpurine cap).
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:mRNA (nucleoside-2'-O-)-methyltransferase. Other names in common use include messenger ribonucleate nucleoside 2'-methyltransferase, and messenger RNA (nucleoside-2'-)-methyltransferase.
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2GA9 and 2GAF.
References
- Barbosa E, Moss B (1978). "mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus Purification and physical properties". J. Biol. Chem. 253 (21): 7692–7. PMID 701281.
- Barbosa E, Moss B (1978). "mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus Characteristics and substrate specificity". J. Biol. Chem. 253 (21): 7698–702. PMID 701282.
- Boone RF, Ensinger MJ, Moss B (1977). "Synthesis of mRNA guanylyltransferase and mRNA methyltransferases in cells infected with vaccinia virus". J. Virol. 21 (2): 475–83. PMC 353847
. PMID 833934.
- Ensinger MJ, Martin SA, Paoletti E, Moss B (1975). "Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus". Proc. Natl. Acad. Sci. U.S.A. 72 (7): 2525–9. doi:10.1073/pnas.72.7.2525. PMC 432801
. PMID 1058472.
- Groner Y, Gilboa E, Aviv H (1978). "Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates". Biochemistry. 17 (6): 977–82. doi:10.1021/bi00599a005. PMID 629955.