Protein N-terminal methyltransferase

Protein N-terminal methyltransferase
Identifiers
EC number	2.1.1.244
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Protein N-terminal methyltransferase (EC 2.1.1.244, NMT1 (gene), METTL11A (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:N-terminal-(A,P,S)PK-(protein) methyltransferase.^[1][2] This enzyme catalyses the following chemical reaction

(1) 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] $\rightleftharpoons$ 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein] (overall reaction)

(1a) S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] $\rightleftharpoons$ S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,S)PK-[protein]

(1b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-(A,S)PK-[protein] $\rightleftharpoons$ S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-(A,S)PK-[protein]

(1c) S-adenosyl-L-methionine + N-terminal-N,N-dimethyl-N-(A,S)PK-serine-[protein] $\rightleftharpoons$ S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein]

(2) 2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] $\rightleftharpoons$ 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein] (overall reaction)

(2a) S-adenosyl-L-methionine + N-terminal-PPK-[protein] $\rightleftharpoons$ S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-PPK-[protein]

(2b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-PPK-[protein] $\rightleftharpoons$ S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein]

This enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys.

References

1. Webb, K.J.; Lipson, R.S.; Al-Hadid, Q.; Whitelegge, J.P.; Clarke, S.G. (2010). "Identification of protein N-terminal methyltransferases in yeast and humans". Biochemistry. 49: 5225–5235. doi:10.1021/bi100428x. PMID 20481588.
2. Tooley, C.E.; Petkowski, J.J.; Muratore-Schroeder, T.L.; Balsbaugh, J.L.; Shabanowitz, J.; Sabat, M.; Minor, W.; Hunt, D.F.; Macara, I.G. (2010). "NRMT is an α-N-methyltransferase that methylates RCC1 and retinoblastoma protein". Nature. 466: 1125–1128. doi:10.1038/nature09343. PMID 20668449.

External links

• Protein N-terminal methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)