S-specific spore photoproduct lyase

Identifiers

Databases

PDB structures

S-specific spore photoproduct lyase (EC 4.1.99.15, SAM, SP lyase, SPL, SplB, SplG) is an enzyme with systematic name S-specific spore photoproduct pyrimidine-lyase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

(5S)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in DNA) + S-adenosyl-L-methionine

\rightleftharpoons

thymidylyl-(3'->5')-thymidylate (in DNA) + 5'-deoxyadenosine + L-methionine

This enzyme is an iron-sulfur protein.

References

↑ Chandor, A.; Berteau, O.; Douki, T.; Gasparutto, D.; Sanakis, Y.; Ollagnier-de-Choudens, S.; Atta, M.; Fontecave, M. (2006). "Dinucleotide spore photoproduct, a minimal substrate of the DNA repair spore photoproduct lyase enzyme from Bacillus subtilis". J. Biol. Chem. 281: 26922–26931. doi:10.1074/jbc.m602297200. PMID 16829676.
↑ Pieck, J.C.; Hennecke, U.; Pierik, A.J.; Friedel, M.G.; Carell, T. (2006). "Characterization of a new thermophilic spore photoproduct lyase from Geobacillus stearothermophilus (SplG) with defined lesion containing DNA substrates". J. Biol. Chem. 281: 36317–36326. doi:10.1074/jbc.m607053200. PMID 16968710.
↑ Mehl, R.A.; Begley, T.P. (1999). "Mechanistic studies on the repair of a novel DNA photolesion: the spore photoproduct". Org Lett. 1: 1065–1066. doi:10.1021/ol9908676. PMID 10825958.
↑ Friedel, M.G.; Berteau, O.; Pieck, J.C.; Atta, M.; Ollagnier-de-Choudens, S.; Fontecave, M.; Carell, T. (2006). "The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion". Chem. Commun. (Camb.): 445–447. doi:10.1039/b514103f. PMID 16493831.

Carbon-carbon lyases (EC 4.1)

4.1.1: Carboxy-lyases	Acetoacetate decarboxylase Adenosylmethionine decarboxylase Aromatic L-amino acid decarboxylase Glutamate decarboxylase Histidine decarboxylase Lysine decarboxylase Malonyl-CoA decarboxylase Ornithine decarboxylase Oxaloacetate decarboxylase Phosphoenolpyruvate carboxykinase Phosphoenolpyruvate carboxylase Phosphoribosylaminoimidazole carboxylase Pyrophosphomevalonate decarboxylase Pyruvate decarboxylase RuBisCO Uridine monophosphate synthetase/Orotidine 5'-phosphate decarboxylase Uroporphyrinogen III decarboxylase

4.1.2: Aldehyde-lyases	Fructose-bisphosphate aldolase Aldolase A Aldolase B Aldolase C 2-hydroxyphytanoyl-CoA lyase Threonine aldolase

4.1.3: Oxo-acid-lyases	Isocitrate lyase 3-hydroxy-3-methylglutaryl-CoA lyase

4.1.99: Other	Tryptophanase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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