Sabin–Feldman dye test

A Sabin–Feldman dye test is a serologic test to diagnose for toxoplasmosis. The test is based on the presence of certain antibodies that prevent methylene blue dye from entering the cytoplasm of Toxoplasma organisms. Patient serum is treated with Toxoplasma trophozoites and complements as activator, and then incubated. After incubation, methylene blue is added. If anti-Toxo antibodies are present in the serum, because these antibodies are activated by complements and lyse the parasite membrane, Toxoplasma trophozoites are not stained (positive result); if there are no antibodies, trophozoites with intact membrane are stained and appear blue under microscope (negative result)

References

Mosby's Medical, Nursing, & Allied Health Dictionary. Edition 5, 1998 p7B4A.

Medical test: Infectious disease blood tests (CPT 87001–87999)

Bacterial infection	syphilis VDRL rapid plasma reagin Wassermann test FTA-ABS Rickettsia Weil–Felix test Helicobacter HelicoCARE direct Streptococcus antistreptolysin O titre

Viral infection	HIV HIV test BDNA test mChip Epstein–Barr virus monospot test Dengue fever NS1 antigen test

Protozoan infection	toxoplasmosis Sabin–Feldman dye test

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