TRNA (pseudouridine54-N1)-methyltransferase

TRNA (pseudouridine⁵⁴-N¹)-methyltransferase (EC 2.1.1.257, TrmY, m1Psi methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (pseudouridine54-N1)-methyltransferase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + pseudouridine⁵⁴ in tRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + N¹-methylpseudouridine⁵⁴ in tRNA

This archaeal enzyme is specific for the 54 position.

References

↑ Chen, H.Y.; Yuan, Y.A. (2010). "Crystal structure of Mj1640/DUF358 protein reveals a putative SPOUT-class RNA methyltransferase". J. Mol. Cell. Biol. 2: 366–374. doi:10.1093/jmcb/mjq034. PMID 21098051.
↑ Wurm, J.P.; Griese, M.; Bahr, U.; Held, M.; Heckel, A.; Karas, M.; Soppa, J.; Wohnert, J. (2012). "Identification of the enzyme responsible for N¹-methylation of pseudouridine 54 in archaeal tRNAs". RNA. 18: 412–420. doi:10.1261/rna.028498.111. PMID 22274954.
↑ Chatterjee, K.; Blaby, I.K.; Thiaville, P.C.; Majumder, M.; Grosjean, H.; Yuan, Y.A.; Gupta, R.; de Crecy-Lagard, V. (2012). "The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at position 54 of tRNA". RNA. 18: 421–433. doi:10.1261/rna.030841.111. PMID 22274953.

External links

TRNA (pseudouridine54-N1)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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