Thiazole synthase

Thiazole synthase
Identifiers
EC number	2.8.1.10
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Thiazole synthase (EC 2.8.1.10, thiG (gene)) is an enzyme with systematic name 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase.^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] $\rightleftharpoons$ 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H₂O

H₂S can provide the sulfur in vitro.

References

1. Park, J.H.; Dorrestein, P.C.; Zhai, H.; Kinsland, C.; McLafferty, F.W.; Begley, T.P. (2003). "Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B₁)". Biochemistry. 42: 12430–12438. doi:10.1021/bi034902z. PMID 14567704.
2. Dorrestein, P.C.; Zhai, H.; McLafferty, F.W.; Begley, T.P. (2004). "The biosynthesis of the thiazole phosphate moiety of thiamin: the sulfur transfer mediated by the sulfur carrier protein ThiS". Chem. Biol. 11: 1373–1381. doi:10.1016/j.chembiol.2004.08.009. PMID 15489164.
3. Dorrestein, P.C.; Zhai, H.; Taylor, S.V.; McLafferty, F.W.; Begley, T.P. (2004). "The biosynthesis of the thiazole phosphate moiety of thiamin (vitamin B₁): the early steps catalyzed by thiazole synthase". J. Am. Chem. Soc. 126: 3091–3096. doi:10.1021/ja039616p. PMID 15012138.
4. Settembre, E.C.; Dorrestein, P.C.; Zhai, H.; Chatterjee, A.; McLafferty, F.W.; Begley, T.P.; Ealick, S.E. (2004). "Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex". Biochemistry. 43: 11647–11657. doi:10.1021/bi0488911. PMID 15362849.
5. Hazra, A.; Chatterjee, A.; Begley, T.P. (2009). "Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction". J. Am. Chem. Soc. 131: 3225–3229. doi:10.1021/ja806752h. PMID 19216519.
6. Hazra, A.B.; Han, Y.; Chatterjee, A.; Zhang, Y.; Lai, R.Y.; Ealick, S.E.; Begley, T.P. (2011). "A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase". J. Am. Chem. Soc. 133: 9311–9319. doi:10.1021/ja1110514. PMID 21534620.

External links

• Thiazole synthase at the US National Library of Medicine Medical Subject Headings (MeSH)