UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting)

UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115, FlaA1, UDP-N-acetylglucosamine 5-inverting 4,6-dehydratase, PseB, UDP-N-acetylglucosamine hydro-lyase (inverting, UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose-forming)) is an enzyme with systematic name UDP-N-acetyl-alpha-D-glucosamine hydro-lyase (inverting; UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose-forming).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

UDP-N-acetyl-alpha-D-glucosamine

\rightleftharpoons

UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H₂O

This enzyme contains NADP⁺ as a cofactor.

References

↑ Ishiyama, N.; Creuzenet, C.; Miller, W.L.; Demendi, M.; Anderson, E.M.; Harauz, G.; Lam, J.S.; Berghuis, A.M. (2006). "Structural studies of FlaA1 from Helicobacter pylori reveal the mechanism for inverting 4,6-dehydratase activity". J. Biol. Chem. 281: 24489–24495. doi:10.1074/jbc.m602393200. PMID 16651261.
↑ Schirm, M.; Soo, E.C.; Aubry, A.J.; Austin, J.; Thibault, P.; Logan, S.M. (2003). "Structural, genetic and functional characterization of the flagellin glycosylation process in Helicobacter pylori". Mol. Microbiol. 48: 1579–1592. doi:10.1046/j.1365-2958.2003.03527.x. PMID 12791140.
↑ Schoenhofen, I.C.; McNally, D.J.; Brisson, J.R.; Logan, S.M. (2006). "Elucidation of the CMP-pseudaminic acid pathway in Helicobacter pylori: synthesis from UDP-N-acetylglucosamine by a single enzymatic reaction". Glycobiology. 16: 8C–14C. doi:10.1093/glycob/cwl010. PMID 16751642.

External links

UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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