Centromere protein E

CENPE
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1T5C
Identifiers
Aliases	CENPE, CENP-E, KIF10, PPP1R61, MCPH13, Centromere protein E
External IDs	MGI: 1098230 HomoloGene: 20429 GeneCards: CENPE
Gene ontology Molecular function • nucleotide binding • ATPase activity • kinetochore binding • microtubule binding • ATP binding • protein binding • microtubule motor activity Cellular component • spindle • cytoplasm • kinesin complex • chromosome • cytosol • nucleus • condensed chromosome kinetochore • condensed chromosome outer kinetochore • chromosome, centromeric region • membrane • midbody • microtubule • kinetochore • condensed chromosome, centromeric region • microtubule cytoskeleton • mitotic spindle midzone • cytoskeleton Biological process • regulation of mitotic metaphase/anaphase transition • cell cycle • mitotic nuclear division • positive regulation of protein kinase activity • multicellular organism development • cell division • antigen processing and presentation of exogenous peptide antigen via MHC class II • mitotic chromosome movement towards spindle pole • kinetochore assembly • microtubule-based movement • attachment of mitotic spindle microtubules to kinetochore • chromosome segregation • mitotic metaphase plate congression • sister chromatid cohesion • retrograde vesicle-mediated transport, Golgi to ER • mitotic cell cycle Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1062	229841
Ensembl	ENSG00000138778	ENSMUSG00000045328
UniProt	Q02224	Q6RT24
RefSeq (mRNA)	NM_001286734 NM_001813	NM_173762
RefSeq (protein)	NP_001273663.1 NP_001804.2	NP_776123.3
Location (UCSC)	Chr 4: 103.11 – 103.2 Mb	Chr 3: 135.21 – 135.27 Mb
PubMed search	[1]	[2]
Wikidata

View/Edit Human

View/Edit Mouse

Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene.^[3][4]

Centromere-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centromere-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation.^[4]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Testa JR, Zhou JY, Bell DW, Yen TJ (Mar 1995). "Chromosomal localization of the genes encoding the kinetochore proteins CENPE and CENPF to human chromosomes 4q24→q25 and 1q32→q41, respectively, by fluorescence in situ hybridization". Genomics. 23 (3): 691–3. doi:10.1006/geno.1994.1558. PMID 7851898.
4. "Entrez Gene: CENPE centromere protein E, 312kDa".

Further reading

• Yen TJ, Li G, Schaar BT, et al. (1992). "CENP-E is a putative kinetochore motor that accumulates just before mitosis". Nature. 359 (6395): 536–9. doi:10.1038/359536a0. PMID 1406971.
• Yen TJ, Compton DA, Wise D, et al. (1991). "CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase". EMBO J. 10 (5): 1245–54. PMC 452779. PMID 2022189.
• Thrower DA, Jordan MA, Schaar BT, et al. (1995). "Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor". EMBO J. 14 (5): 918–26. PMC 398164. PMID 7889940.
• Wood KW, Sakowicz R, Goldstein LS, Cleveland DW (1997). "CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment". Cell. 91 (3): 357–66. doi:10.1016/S0092-8674(00)80419-5. PMID 9363944.
• Cooke CA, Schaar B, Yen TJ, Earnshaw WC (1998). "Localization of CENP-E in the fibrous corona and outer plate of mammalian kinetochores from prometaphase through anaphase". Chromosoma. 106 (7): 446–55. doi:10.1007/s004120050266. PMID 9391217.
• Zecevic M, Catling AD, Eblen ST, et al. (1998). "Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E". J. Cell Biol. 142 (6): 1547–58. doi:10.1083/jcb.142.6.1547. PMC 2141767. PMID 9744883.
• Chan GK, Schaar BT, Yen TJ (1998). "Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1". J. Cell Biol. 143 (1): 49–63. doi:10.1083/jcb.143.1.49. PMC 2132809. PMID 9763420.
• Chan GK, Jablonski SA, Sudakin V, et al. (1999). "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC". J. Cell Biol. 146 (5): 941–54. doi:10.1083/jcb.146.5.941. PMC 2169490. PMID 10477750.
• Ashar HR, James L, Gray K, et al. (2000). "Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules". J. Biol. Chem. 275 (39): 30451–7. doi:10.1074/jbc.M003469200. PMID 10852915.
• Uren AG, Wong L, Pakusch M, et al. (2001). "Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype". Curr. Biol. 10 (21): 1319–28. doi:10.1016/S0960-9822(00)00769-7. PMID 11084331.
• Suzuki Y, Tsunoda T, Sese J, et al. (2001). "Identification and characterization of the potential promoter regions of 1031 kinds of human genes". Genome Res. 11 (5): 677–84. doi:10.1101/gr.164001. PMC 311086. PMID 11337467.
• Liu ST, Chan GK, Hittle JC, et al. (2003). "Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores". Mol. Biol. Cell. 14 (4): 1638–51. doi:10.1091/mbc.02-05-0074. PMC 153128. PMID 12686615.
• Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
• Garcia-Saez I, Blot D, Kahn R, Kozielski F (2005). "Crystallization and preliminary crystallographic analysis of the motor domain of human kinetochore-associated protein CENP-E using an automated crystallization procedure". Acta Crystallogr. D. 60 (Pt 6): 1158–60. doi:10.1107/S0907444904009564. PMID 15159587.
• Tanudji M, Shoemaker J, L'Italien L, et al. (2005). "Gene silencing of CENP-E by small interfering RNA in HeLa cells leads to missegregation of chromosomes after a mitotic delay". Mol. Biol. Cell. 15 (8): 3771–81. doi:10.1091/mbc.E03-07-0482. PMC 491836. PMID 15181147.
• Garcia-Saez I, Yen T, Wade RH, Kozielski F (2004). "Crystal structure of the motor domain of the human kinetochore protein CENP-E". J. Mol. Biol. 340 (5): 1107–16. doi:10.1016/j.jmb.2004.05.053. PMID 15236970.
• Kurasawa Y, Earnshaw WC, Mochizuki Y, et al. (2005). "Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation". EMBO J. 23 (16): 3237–48. doi:10.1038/sj.emboj.7600347. PMC 514520. PMID 15297875.
• Mao Y, Desai A, Cleveland DW (2005). "Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling". J. Cell Biol. 170 (6): 873–80. doi:10.1083/jcb.200505040. PMC 2171436. PMID 16144904.
• Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.