Viral infectivity factor

Viral infectivity factor, or Vif, is a protein found in HIV and other retroviruses. Its role is to disrupt the antiviral activity of the human enzyme APOBEC (See also APOBEC3G) by targeting it for ubiquitination and cellular degradation. APOBEC is a cytidine deaminase enzyme that mutates viral nucleic acids.

Ribbon representation of Crystal Structure of the HIV Vif BC-box in Complex with Human ElonginB and ElonginC[1]

Vif is a 23-kilodalton protein that is essential for viral replication. Vif inhibits the cellular protein, APOBEC3G, from entering the virion during budding from a host cell by targeting it for proteasomal degradation. Vif hijacks the cellular Cullin5 E3 ubiquitin ligase, which is composed of ElonginB, ElonginC, Cullin5, and Rbx2, (Crystal Structure of the HIV Vif BC-box in Complex with Human ElonginB and ElonginC is solved and shown here)[1] in order to target APOBEC3G for degradation. In the absence of Vif, APOBEC3G causes hypermutation of the viral genome, rendering it dead-on-arrival at the next host cell. APOBEC3G is thus a host defence to retroviral infection which HIV-1 has overcome by the acquisition of Vif. Targeting vif has been suggested as a strategy for future HIV drug therapies.[2]

Vif was considered as a phospho-protein and phosphorylation seemed to be required for viral infectivity.[3][4] But recent studies with the use of metabolic labelling demonstrated that serine/threonine phosphorylation of Vif and A3G is not required for the interaction of Vif with A3G for Vif dependent degradation of A3G and the antiviral activity of A3G.[5]

References

  1. 1 2 Stanley, BJ; Ehrlich, E.S.; Short, L.; et al. (18 June 2008). "Structural insight into the human immunodeficiency virus Vif SOCS box and its role in human E3 ubiquitin ligase assembly". J. Virol. 82: 8656–63. doi:10.1128/JVI.00767-08. PMC 2519636Freely accessible. PMID 18562529.; rendered with PyMOL
  2. ↑ Miller JH, Presnyak V, Smith HC (2007). "The dimerization domain of HIV-1 viral infectivity factor Vif is required to block APOBEC3G incorporation with virions". Retrovirology. 4 (1): 81. doi:10.1186/1742-4690-4-81. PMC 2222665Freely accessible. PMID 18036235.
  3. ↑ Yang, X; Gabuzda D (1998). "Mitogen-activated protein kinase phosphorylates and regulates the HIV-1 Vif protein.". J. Biol. Chem. 273 (45): 29879–87. doi:10.1074/jbc.273.45.29879. PMID 9792705.
  4. ↑ Yang, X; Goncalves J; Gabuzda D. (1996). "Phosphorylation of Vif and its role in HIV-1 replication.". J. Biol. Chem. 271 (17): 10121–9. PMID 8626571.
  5. ↑ Kopietz F, Jaguva Vasudevan AA, Krämer M, Muckenfuss H, Sanzenbacher R, Cichutek K, Flory E, Münk C (15 August 2012). "Interaction of human immunodeficiency virus type 1 Vif with APOBEC3G is not dependent on serine/threonine phosphorylation status.". J Gen Virol. 93 (11): 2425–30. doi:10.1099/vir.0.043273-0. PMID 22894923.

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