Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase
| galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.4.1.135 | ||||||||
| CAS number | 9030-08-4 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / EGO | ||||||||
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In enzymology, a galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase (EC 2.4.1.135) is an enzyme that catalyzes the chemical reaction
- UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O- beta-D-xylosylprotein
Thus, the two substrates of this enzyme are UDP-glucuronate and 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein, whereas its 3 products are UDP, 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-, and beta-D-xylosylprotein.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-glucuronate:3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xyl osyl-protein D-glucuronosyltransferase. Other names in common use include glucuronosyltransferase I, and uridine diphosphate glucuronic acid:acceptor glucuronosyltransferase. This enzyme participates in chondroitin sulfate biosynthesis and glycan structures - biosynthesis 1. It employs one cofactor, manganese.
Structural studies
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1V82, 1V83, 1V84, and 2D0J.
References
- Helting T, Roden L (1969). "Biosynthesis of chondroitin sulfate. II. Glucuronosyl transfer in the formation of the carbohydrate-protein linkage region". J. Biol. Chem. 244 (10): 2799–805. PMID 5770003.
- Helting T (1972). "Biosynthesis of heparin. Solubilization and partial purification of uridine diphosphate glucuronic acid: acceptor glucuronosyltransferase from mouse mastocytoma". J. Biol. Chem. 247 (13): 4327–32. PMID 4260846.
- T, Sugahara K; Tone, Y; Tamura, J; Neumann, KW; Ogawa, T; Oka, S; Kawasaki, T; Sugahara, K (1998). "Molecular cloning and expression of glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans". J. Biol. Chem. 273 (12): 6615–8. doi:10.1074/jbc.273.12.6615. PMID 9506957.