Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase
| Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.4.2.43 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase (EC 2.4.2.43, undecaprenyl phosphate-alpha-L-Ara4N transferase, 4-amino-4-deoxy-L-arabinose lipid A transferase, polymyxin resistance protein PmrK, arnT (gene)) is an enzyme with systematic name 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate:lipid IVA 4-amino-4-deoxy-L-arabinopyranosyltransferase.[1][2][3][4][5] This enzyme catalyses the following chemical reaction
- (1) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans,octacis-undecaprenyl phosphate
- (2) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + lipid IVA lipid IIA + ditrans,octacis-undecaprenyl phosphate
- (3) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA 4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + ditrans,octacis-undecaprenyl phosphate
This integral membrane protein is present in the inner membrane of certain Gram negative endobacteria.
References
- ↑ Trent, M.S.; Ribeiro, A.A.; Lin, S.; Cotter, R.J.; Raetz, C.R. (2001). "An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor". J. Biol. Chem. 276: 43122–43131. doi:10.1074/jbc.m106961200. PMID 11535604.
- ↑ Trent, M.S.; Ribeiro, A.A.; Doerrler, W.T.; Lin, S.; Cotter, R.J.; Raetz, C.R. (2001). "Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm". J. Biol. Chem. 276: 43132–43144. doi:10.1074/jbc.m106962200. PMID 11535605.
- ↑ Zhou, Z.; Ribeiro, A.A.; Lin, S.; Cotter, R.J.; Miller, S.I.; Raetz, C.R. (2001). "Lipid A modifications in polymyxin-resistant Salmonella typhimurium: PMRA-dependent 4-amino-4-deoxy-L-arabinose, and phosphoethanolamine incorporation". J. Biol. Chem. 276: 43111–43121. doi:10.1074/jbc.m106960200. PMID 11535603.
- ↑ Bretscher, L.E.; Morrell, M.T.; Funk, A.L.; Klug, C.S. (2006). "Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium". Protein Expr. Purif. 46: 33–39. doi:10.1016/j.pep.2005.08.028. PMID 16226890.
- ↑ Impellitteri, N.A.; Merten, J.A.; Bretscher, L.E.; Klug, C.S. (2010). "Identification of a functionally important loop in Salmonella typhimurium ArnT". Biochemistry. 49: 29–35. doi:10.1021/bi901572f. PMID 19947657.
External links
- Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)
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