Mannose

Mannose

D-Mannopyranose
Fischer projections
Identifiers
CAS Number	3458-28-4
ChEMBL	ChEMBL469448
ChemSpider	17893 D-mannopyranose
IUPHAR/BPS	4650
MeSH	Mannose
PubChem	18950
UNII	PHA4727WTP
Properties
Chemical formula	C6H12O6
Molar mass	180.16 g·mol^−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Mannose, packaged as the nutritional supplement "d-mannose", is a sugar monomer of the aldohexose series of carbohydrates. Mannose is a C-2 epimer of glucose. Mannose is important in human metabolism, especially in the glycosylation of certain proteins. Several congenital disorders of glycosylation are associated with mutations in enzymes involved in mannose metabolism.^[1]

Mannose is not an essential nutrient; it can be produced in the human body from glucose, or converted into glucose. Mannose provides 2-5 kilocalories per gram of energy. Mannose is partially excreted in the urine.

Structure

Mannose commonly exists as two different sized rings, the pyranose (six-membered) form and the furanose (five-membered) form. Each ring closure can have either an alpha or beta configuration at the anomeric position. The chemical rapidly undergoes isomerization among these four forms.

D-Mannose isomers (Haworth projections)
Percent composition

α-D-Mannofuranose <1 %	β-D-Mannofuranose <1 %
α-D-Mannopyranose 67 %	β-D-Mannopyranose 33 %

Metabolism

Metabolism of common monosaccharides and related reactions

While much of the mannose used in glycosylation is believed to be derived from glucose, in cultured hepatoma (cancerous cells from the liver) cells, most of the mannose for glycoprotein biosynthesis comes from extracellular mannose, not glucose.^[2] Many of the glycoproteins produced in the liver are secreted into the bloodstream, so dietary mannose is distributed throughout the body. ^[3]

Mannose is present in numerous glycoconjugates including N-linked glycosylation of proteins. C-Mannosylation is also abundant and can be found in collagen-like regions.

The digestion of many polysaccharides and glycoproteins yields mannose which is phosphorylated by hexokinase to generate mannose-6-phosphate. Mannose-6-phosphate is converted to fructose-6-phosphate, by the enzyme phosphomannose isomerase, and then enters the glycolytic pathway or is converted to glucose-6-phosphate by the gluconeogenic pathway of hepatocytes.

Mannose is a dominant monosaccharide in N-linked glycosylation. N-Linked glycosylation is a post-translational modification of proteins. It is initiated by the en bloc transfer on Glc3Man9GlcNAc2 to nascent glycoproteins in the endoplasmic reticulum in a co-translational manner as the protein entered through the transport system. Glucose is hydrolyzed on fully folded protein and the mannose moieties are hydrolyzed by ER and Golgi-resident mannosidases. Typically, mature human glycoproteins only contain three mannose residues buried under sequential modification by GlcNAc, galactose and sialic acid. This is important as the innate immune system in mammals is geared to recognise exposed mannose residues. This activity is due to the prevalence of mannose residues, in the form of mannans, on the surface of yeast. The human immunodeficiency virus displays considerable amount of mannose residues due to the tight clustering of glycans in its viral spike.^[4][5] These mannose residues are the target for broadly neutralizing antibodies.^[6]

Biotechnology

Recombinant proteins produced in yeast may be subject to mannose addition in patterns different from those used by mammalian cells.^[7] This difference in recombinant proteins from those normally produced in mammalian organisms may influence the effectiveness of vaccines.

Formation

Mannose can be formed by the oxidation of mannitol.

It can also be formed from glucose in the Lobry-de Bruyn-van Ekenstein transformation.

Etymology

The root of both "mannose" and "mannitol" is manna, which the Bible records as the food supplied to the Israelites during their journey in the region of Sinai. Several trees and shrubs can produce a substance called manna, such as the "manna tree" (Fraxinus ornus) from whose secretions mannitol was originally isolated.

Configuration

Mannose differs from glucose by inversion of the C-2 chiral center. Mannose displays a $^{4}C_{1}$ pucker in the solution ring form. This simple change leads to the drastically different biochemistry of the two hexoses. This change has the same effect on the other aldohexoses as well.

Mannose PTS permease

Mannose XYZ permease complex: entry of PEP which donates a high energy phosphate that gets passed through the transporter system and eventually assist in the entry of mannose (in this example otherwise it would any hexose sugar) and results in the formation of mannose-6-phosphate.

Video illustration of the MANXYZ sugar transporter complex transferring the high energy phosphate for PEP to the other subunits of the complex

The PEP-dependent sugar transporting phosphotransferase system transports and simultaneously phosphorylates its sugar substrates. Mannose XYZ permease is a member of the family, with this distinct method being used by bacteria for sugar uptake particularly exogenous hexoses in the case of Mannose XYZ in order to release the phosphate esters into the cell cytoplasm in preparation for metabolism primarily through the route of glycolysis.^[8] The MANXYZ transporter complex is also involved in infection of E. Coli by bacteriophage Lambda, with subunit ManX and ManY being sufficient for proper Lambda phage infection.^[9] MANXYZ possesses four domains in three polypeptide chains; ManX, ManY, and ManZ. The ManX subunit forms a homodimer that is localized to the cytoplasmic side of the membrane. ManX contains two domains IIA and IIB linked by a hinge peptide with each domain containing a phosphorylation site and phosphoryl transfer occurs between both subunits.^[10] ManX can be membrane bound or non-membrane bound.^[9] The MANY and MANZ subunits are hydrophobic integral membrane proteins with six and one transmembrane alpha helical spanner(s).^[11] The phosphoryl group of PEP is transferred to the imported sugar via E1 (Enzyme 1), HPr (Histidine Protein) phosphate carrier and then to the MANX, MANY, and MANZ subunits of the MANXYZ transportation complex which phosphorylate the entering hexose sugar creating a hexose-6-phosphate.

Therapeutic potential

Preliminary studies indicate that D-mannose may help to prevent recurrent urinary tract infections.^[12]

See also

• α-Mannosidase
• Mannose receptor
• Mannan oligosaccharide-based nutritional supplements
• Rhamnose, 6-deoxy-L-mannose

References

1. Freeze, H. H.; Sharma, V. (2010). "Metabolic manipulation of glycosylation disorders in humans and animal models". Seminars in Cell & Developmental Biology. 21 (6): 655–662. doi:10.1016/j.semcdb.2010.03.011. PMC 2917643. PMID 20363348.
2. Alton, G.; Hasilik, M.; Niehues, R.; Panneerselvam, K.; Etchison, J. R.; Fana, F.; Freeze, H. H. (1998). "Direct utilization of mannose for mammalian glycoprotein biosynthesis". Glycobiology. 8 (3): 285–295. doi:10.1093/glycob/8.3.285. PMID 9451038.
3. Davis, J. A.; Freeze, H. H. (2001). "Studies of mannose metabolism and effects of long-term mannose ingestion in the mouse". Biochimica et Biophysica Acta. 1528 (2–3): 116–126. doi:10.1016/S0304-4165(01)00183-0. PMID 11687298.
4. Pritchard, Laura K.; Spencer, Daniel I. R.; Royle, Louise; Bonomelli, Camille; Seabright, Gemma E.; Behrens, Anna-Janina; Kulp, Daniel W.; Menis, Sergey; Krumm, Stefanie A. (2015-06-24). "Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies". Nature Communications. 6: 7479. doi:10.1038/ncomms8479. PMC 4500839. PMID 26105115.
5. Pritchard, Laura K.; Vasiljevic, Snezana; Ozorowski, Gabriel; Seabright, Gemma E.; Cupo, Albert; Ringe, Rajesh; Kim, Helen J.; Sanders, Rogier W.; Doores, Katie J. (2015-06-16). "Structural Constraints Determine the Glycosylation of HIV-1 Envelope Trimers". Cell Reports. 11 (10): 1604–1613. doi:10.1016/j.celrep.2015.05.017. ISSN 2211-1247. PMC 4555872. PMID 26051934.
6. Crispin, Max; Doores, Katie J (2015-04-01). "Targeting host-derived glycans on enveloped viruses for antibody-based vaccine design". Current Opinion in Virology. Viral pathogenesis • Preventive and therapeutic vaccines. 11: 63–69. doi:10.1016/j.coviro.2015.02.002.
7. Vlahopoulos, S.; Gritzapis, A. D.; Perez, S. A.; Cacoullos, N.; Papamichail, M.; Baxevanis, C. N. (2009). "Mannose addition by yeast Pichia Pastoris on recombinant HER-2 protein inhibits recognition by the monoclonal antibody herceptin". Vaccine. 27 (34): 4704–4708. doi:10.1016/j.vaccine.2009.05.063. PMID 19520203.
8. Postma, P. W.; Lengeler, J. W.; Jacobson, G. R. (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria". Microbiological reviews. 57 (3): 543–594. PMC 372926. PMID 8246840.
9. Erni, B.; Zanolari, B. (1985). "The mannose-permease of the bacterial phosphotransferase system. Gene cloning and purification of the enzyme IIMan/IIIMan complex of Escherichia coli". The Journal of Biological Chemistry. 260 (29): 15495–15503. PMID 2999119.
10. Erni, B.; Zanolari, B.; Graff, P.; Kocher, H. P. (1989). "Mannose permease of Escherichia coli. Domain structure and function of the phosphorylating subunit". The Journal of Biological Chemistry. 264 (31): 18733–18741. PMID 2681202.
11. Huber, F.; Erni, B. (1996). "Membrane topology of the mannose transporter of Escherichia coli K12". European Journal of Biochemistry / FEBS. 239 (3): 810–817. doi:10.1111/j.1432-1033.1996.0810u.x. PMID 8774730.
12. Kranjčec, Bojana; Papeš, Dino; Altarac, Silvio (2013). "d-mannose powder for prophylaxis of recurrent urinary tract infections in women: a randomized clinical trial". World Journal of Urology. 32 (1): 79–84. doi:10.1007/s00345-013-1091-6. ISSN 0724-4983.