| City |
Country |
IATA |
ICAO |
Airport |
Notes |
| Alicante | Spain | ALC | LEAL | Alicante Airport | Terminated |
| Amsterdam | Netherlands | AMS | EHAM | Amsterdam Airport Schiphol | Terminated |
| Arauca | Colombia | AUC | SKUC | Santiago Pérez Airport | Terminated |
| Armenia | Colombia | AXM | SKAR | El Edén International Airport | |
| Bahía Solano | Colombia | BSB | SKBS | José Celestino Mutis Airport | Terminated |
| Barcelona | Spain | BCN | LEBL | Barcelona El Prat Airport | |
| Barrancabermeja | Colombia | EJA | SKEJ | Yariguíes Airport | |
| Barranquilla | Colombia | BAQ | SKBQ | Ernesto Cortissoz International Airport | |
| Bogotá | Colombia | BOG | SKBO | El Dorado International Airport | |
| Bridgetown | Barbados | BGI | TBPB | Grantley Adams International Airport | [1] |
| Bucaramanga | Colombia | BGA | SKBG | Palonegro International Airport | |
| Buenos Aires | Argentina | EZE | SAEZ | Ministro Pistarini International Airport | |
| Cali | Colombia | CLO | SKCL | Alfonso Bonilla Aragón International Airport | |
| Cancún | Mexico | CUN | MMUN | Cancún International Airport | |
| Caracas | Venezuela | CCS | SVMI | Simon Bolivar International Airport | |
| Cartagena | Colombia | CTG | SKCG | Rafael Núñez International Airport | |
| Cúcuta | Colombia | CUC | SKCC | Camilo Daza International Airport | |
| Florencia | Colombia | FLA | SKFL | Gustavo Artunduaga Paredes Airport | |
| Fort Lauderdale | United States | FLL | KFLL | Fort Lauderdale-Hollywood International Airport | |
| Frankfurt | Germany | FRA | EDDF | Frankfurt am Main Airport | Terminated |
| Guatemala City | Guatemala | GUA | MGGT | La Aurora International Airport | |
| Guayaquil | Ecuador | GYE | SEGU | José Joaquín de Olmedo International Airport | Operated by Avianca Ecuador |
| Havana | Cuba | HAV | MUHA | José Martí International Airport | |
| Ibagué | Colombia | IBE | SKIB | Perales Airport | |
| La Paz | Bolivia | LPB | SLLP | El Alto International Airport | |
| Leticia | Colombia | LET | SKLT | Alfredo Vásquez Cobo International Airport | |
| Lima | Peru | LIM | SPIM | Jorge Chávez International Airport | |
| London | United Kingdom | LHR | EGLL | London Heathrow Airport | [2] |
| London | United Kingdom | LGW | EGKK | Gatwick Airport | Terminated |
| Los Angeles | United States | LAX | KLAX | Los Angeles International Airport | [3] |
| Madrid | Spain | MAD | LEMD | Madrid-Barajas Airport | |
| Manaus | Brazil | MAO | SBEG | Eduardo Gomes International Airport | Seasonal Charter |
| Manizales | Colombia | MZL | SKMZ | La Nubia Airport | |
| Medellín | Colombia | EOH | SKMD | Enrique Olaya Herrera Airport | Terminated |
| Medellín | Colombia | MDE | SKRG | José María Córdova International Airport | |
| Mexico City | Mexico | MEX | MMMX | Mexico City International Airport | |
| Miami | United States | MIA | KMIA | Miami International Airport | |
| Montego Bay | Jamaica | MBJ | MKJS | Sangster International Airport | Seasonal Charter |
| Montería | Colombia | MTR | SKMR | Los Garzones Airport | |
| Montevideo | Uruguay | MVD | SUMU | Carrasco International Airport | Terminated |
| Nassau | Bahamas | NAS | CNAS | Lynden Pindling International Airport | Seasonal Charter |
| Neiva | Colombia | NVA | SKNV | Benito Salas Airport | |
| New York | United States | JFK | KJFK | John F. Kennedy International Airport | |
| Nuquí | Colombia | NQU | SKNQ | Reyes Murillo Airport | Terminated |
| Oranjestad | Aruba | AUA | TNCA | Reina Beatrix International Airport | |
| Orlando | United States | MCO | KMCO | Orlando International Airport | |
| Panama City | Panama | PTY | MPTO | Tocumen International Airport | |
| Paris | France | CDG | LFPG | Charles de Gaulle Airport | Terminated |
| Paris | France | ORY | LFPO | Paris-Orly Airport | Terminated |
| Pasto | Colombia | PSO | SKPS | Antonio Nariño Airport | |
| Pereira | Colombia | PEI | SKPE | Matecaña International Airport | |
| Philipsburg | Sint Maarten | SXM | TNCM | Princess Juliana International Airport | Seasonal Charter |
| Popayán | Colombia | PPN | SKPP | Guillermo León Valencia Airport | |
| Porlamar | Venezuela | PMV | SVMG | Del Caribe "Santiago Mariño" International Airport | Seasonal Charter |
| Providencia Island | Colombia | PVA | SKPV | El Embrujo Airport | Terminated |
| Punta Cana | Dominican Republic | PUJ | MDPC | Punta Cana International Airport | |
| Quibdó | Colombia | UIB | SKUI | El Caraño Airport | Terminated |
| Quito | Ecuador | UIO | SEQU | Mariscal Sucre International Airport | |
| Rio de Janeiro | Brazil | GIG | SBGL | Galeão-Antonio Carlos Jobim International Airport | |
| Riohacha | Colombia | RCH | SKRH | Almirante Padilla Airport | |
| Rome | Italy | FCO | LIRF | Rome-Fiumicino Airport | Terminated |
| San Andrés Island | Colombia | ADZ | SKSP | Gustavo Rojas Pinilla International Airport | |
| San José de Costa Rica | Costa Rica | SJO | MROC | Juan Santamaría International Airport | |
| San Juan | Puerto Rico | SJU | TJSJ | Luis Muñoz Marín International Airport | |
| San Salvador | El Salvador | SAL | MSLP | Monseñor Óscar Arnulfo Romero International Airport | Operated by Avianca El Salvador |
| Santa Cruz | Bolivia | VVI | SLVR | Viru Viru International Airport | Terminated |
| Santa Marta | Colombia | SMR | SKSM | Simón Bolívar International Airport | |
| Santiago de Chile | Chile | SCL | SCEL | Comodoro Arturo Merino Benítez International Airport | |
| Santo Domingo | Dominican Republic | SDQ | MDSD | Las Américas International Airport | |
| São Paulo | Brazil | GRU | SBGR | Guarulhos International Airport | |
| Stockholm | Sweden | ARN | ESSA | Stockholm Arlanda Airport | Terminated |
| Tumaco | Colombia | TCO | SKCO | La Florida Airport | |
| Valencia | Venezuela | VLN | SVVA | Arturo Michelena International Airport | Terminated |
| Valencia | Spain | VLC | LEVC | Valencia International Airport | Terminated |
| Valledupar | Colombia | VUP | SKVP | Alfonso López Pumarejo Airport | |
| Varadero | Cuba | VRA | MUVR | Juan Gualberto Gómez Airport | Seasonal Charter |
| Villavicencio | Colombia | VVC | SKVV | La Vanguardia Airport | |
| Washington D.C. | United States | IAD | KIAD | Washington-Dulles International Airport | |
| Willemstad | Curaçao | CUR | TNCC | Hato International Airport | |
| Yopal | Colombia | EYP | SKYP | El Alcaraván Airport | |
| Zurich | Switzerland | ZRH | LSZH | Zurich International Airport | Terminated |
