MYLK

MYLK
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2CQV, 2K0F, 2YR3
Identifiers
Aliases	MYLK, AAT7, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK1, smMLCK, myosin light chain kinase
External IDs	MGI: 894806 HomoloGene: 14202 GeneCards: MYLK
Gene ontology Molecular function • transferase activity • nucleotide binding • protein kinase activity • metal ion binding • calmodulin binding • myosin light chain kinase activity • kinase activity • protein serine/threonine kinase activity • protein binding • actin binding • ATP binding Cellular component • cytoplasm • cytosol • cell projection • stress fiber • cleavage furrow • extracellular exosome • cytoskeleton • lamellipodium Biological process • smooth muscle contraction • muscle contraction • phosphorylation • tonic smooth muscle contraction • positive regulation of cell migration • positive regulation of wound healing • bleb assembly • cellular hypotonic response • protein phosphorylation • aorta smooth muscle tissue morphogenesis • xenophagy • positive regulation of calcium ion transport • positive regulation of defense response to virus by host Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4638	107589
Ensembl	ENSG00000065534	ENSMUSG00000022836
UniProt	Q15746	Q6PDN3
RefSeq (mRNA)	NM_005965 NM_053025 NM_053026 NM_053027 NM_053028 NM_053029 NM_053030 NM_053031 NM_053032 NM_001321309	NM_139300
RefSeq (protein)	NP_444253.3 NP_444254.3 NP_444255.3 NP_444256.3 NP_444259.1 NP_444260.1 NP_001308238.1	n/a
Location (UCSC)	Chr 3: 123.61 – 123.88 Mb	Chr 16: 34.75 – 35 Mb
PubMed search	[1]	[2]
Wikidata

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Myosin light chain kinase, smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene.^[3]

Function

This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.^[4]

See also

• Myosin light-chain kinase

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Potier MC, Chelot E, Pekarsky Y, Gardiner K, Rossier J, Turnell WG (Mar 1996). "The human myosin light chain kinase (MLCK) from hippocampus: cloning, sequencing, expression, and localization to 3qcen-q21". Genomics. 29 (3): 562–70. doi:10.1006/geno.1995.9965. PMID 8575746.
4. "Entrez Gene: MYLK myosin, light chain kinase".

Further reading

• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Clayburgh DR, Rosen S, Witkowski ED, et al. (2005). "A Differentiation-dependent Splice Variant of Myosin Light Chain Kinase, MLCK1, Regulates Epithelial Tight Junction Permeability". J. Biol. Chem. 279 (53): 55506–13. doi:10.1074/jbc.M408822200. PMC 1237105. PMID 15507455.
• Wang F, Graham WV, Wang Y, et al. (2005). "Interferon-γ and Tumor Necrosis Factor-α Synergize to Induce Intestinal Epithelial Barrier Dysfunction by Up-Regulating Myosin Light Chain Kinase Expression". Am. J. Pathol. 166 (2): 409–19. doi:10.1016/S0002-9440(10)62264-X. PMC 1237049. PMID 15681825.
• Russo JM, Florian P, Shen L, et al. (2005). "Distinct Temporal-Spatial Roles for Rho Kinase and Myosin Light Chain Kinase in Epithelial Purse-String Wound Closure". Gastroenterology. 128 (4): 987–1001. doi:10.1053/j.gastro.2005.01.004. PMC 1237051. PMID 15825080.
• Shimizu S, Yoshida T, Wakamori M, et al. (2006). "Ca2+–calmodulin-dependent myosin light chain kinase is essential for activation of TRPC5 channels expressed in HEK293 cells". J. Physiol. (Lond.). 570 (Pt 2): 219–35. doi:10.1113/jphysiol.2005.097998. PMC 1464317. PMID 16284075.
• Kim MT, Kim BJ, Lee JH, et al. (2006). "Involvement of calmodulin and myosin light chain kinase in activation of mTRPC5 expressed in HEK cells". Am. J. Physiol., Cell Physiol. 290 (4): C1031–40. doi:10.1152/ajpcell.00602.2004. PMID 16306123.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Connell LE, Helfman DM (2007). "Myosin light chain kinase plays a role in the regulation of epithelial cell survival". J. Cell. Sci. 119 (Pt 11): 2269–81. doi:10.1242/jcs.02926. PMID 16723733.
• Seguchi O, Takashima S, Yamazaki S, et al. (2007). "A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart". J. Clin. Invest. 117 (10): 2812–24. doi:10.1172/JCI30804. PMC 1978424. PMID 17885681.

External links

• MYLK protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.