Muscarinic acetylcholine receptor M2

CHRM2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3UON, 4MQS, 4MQT
Identifiers
Aliases	CHRM2, HM2, cholinergic receptor muscarinic 2
External IDs	MGI: 88397 HomoloGene: 20190 GeneCards: CHRM2
Targeted by Drug
acetylcholine, arecoline, bethanechol, xanomeline, pilocarpine, brucine, vinburnine, vincamine, staurosporine, aclidinium, AFDX384, amitriptyline, biperiden, dexetimide, dicyclomine, hexocyclium, himbacine, imipramine, methoctramine free base, oxybutynin, pirenzepine, propantheline, clidinium, 3-quinuclidinyl-benzilate, umeclidinium, glycopyrrolate, darifenacin, tolterodine, oxotremorine[1]
Gene ontology Molecular function • G-protein coupled receptor activity • signal transducer activity • drug binding • G-protein coupled acetylcholine receptor activity Cellular component • axon terminus • integral component of membrane • postsynaptic membrane • membrane • plasma membrane • synapse • integral component of plasma membrane • cell junction • neuronal cell body • dendrite • symmetric synapse • asymmetric synapse Biological process • G-protein coupled acetylcholine receptor signaling pathway • G-protein coupled receptor signaling pathway • regulation of heart contraction • regulation of smooth muscle contraction • adenylate cyclase-modulating G-protein coupled receptor signaling pathway • G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger • response to virus • synaptic transmission, cholinergic • nervous system development • adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway • phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway • signal transduction Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	1129	243764
Ensembl	ENSG00000181072	ENSMUSG00000045613
UniProt	P08172	Q9ERZ4
RefSeq (mRNA)	NM_000739 NM_001006626 NM_001006627 NM_001006628 NM_001006629 NM_001006630 NM_001006631 NM_001006632 NM_001006633	NM_203491
RefSeq (protein)	NP_000730.1 NP_001006627.1 NP_001006628.1 NP_001006629.1 NP_001006630.1 NP_001006631.1 NP_001006632.1 NP_001006633.1	NP_987076.2
Location (UCSC)	Chr 7: 136.87 – 137.02 Mb	Chr 6: 36.39 – 36.53 Mb
PubMed search	[2]	[3]
Wikidata

View/Edit Human

View/Edit Mouse

The muscarinic acetylcholine receptor M₂, also known as the cholinergic receptor, muscarinic 2, is a muscarinic acetylcholine receptor that in humans is encoded by the CHRM2 gene.^[4] Multiple alternatively spliced transcript variants have been described for this gene.^[4]

Function

Heart

The M₂ muscarinic receptors are located in the heart, where they act to slow the heart rate down to normal sinus rhythm after positive stimulatory actions of the parasympathetic nervous system, by slowing the speed of depolarization. They also reduce contractile forces of the atrial cardiac muscle, and reduce conduction velocity of the atrioventricular node (AV node). However, they have no effect on the contractile forces of the ventricular muscle.

IQ

A Dutch family study found that there is "a highly significant association" between the CHRM2 gene and intelligence as measured by the Wechsler Adult Intelligence Scale-Revised.^[5] A similar association was found independently in the Minnesota Twin and Family Study.^[6][7]

However, a larger 2009 study attempting to replicate this claim instead found no significant association between the CHRM2 gene and intelligence.^[8]

Olfactory behavior

Mediating olfactory guided behaviors (e.g. odor discrimination, aggression, mating) ^[9]

Mechanism of action

M₂ muscarinic receptors act via a G_i type receptor, which causes a decrease in cAMP in the cell, generally leading to inhibitory-type effects. They appear to serve as autoreceptors.^[10]

In addition, they modulate muscarinic potassium channels.^[11][12] In the heart, this contributes to a decreased heart rate. They do so by the G beta gamma subunit of the G protein coupled to M₂. This part of the G protein can open K⁺ channels in the parasympathetic notches in the heart, which causes an outward current of potassium, which slows down the heart rate.

Ligands

Few highly selective M₂ agonists are available at present, although there are several non-selective muscarinic agonists that stimulate M₂, and a number of selective M₂ antagonists are available.

Agonists

• methacholine (selective m2 muscarinic agonist)
• (2S,2'R,3'S,5'R)-1-methyl-2-(2-methyl-1,3-oxathiolan-5-yl)pyrrolidine 3-sulfoxide methyl iodide (selective for M₂ but only partial agonist)^[13]
• Berberine

Antagonists

• Atropine^[11]
• Hyoscyamine^[14]
• Dimethindene - N,N-Dimethyl-3-[(1S)-1-(2-pyridinyl)ethyl]-1H-indene-2-ethanamine, CAS# 121367-05-3, mixed M₂ / histamine H₁ antagonist
• Otenzepad - 11-([2-[(Diethylamino)methyl]-1-piperidinyl]acetyl)-5,11-dihydro-6H-pyrido[2,3-b][1,4]benzodiazepin-6-one, CAS# 102394-31-0
• AQRA-741 - 11-([4-[4-(Diethylamino)butyl]-1-piperidinyl]acetyl)-5,11-dihydro-6H-pyrido[2,3-b][1,4]benzodiazepin-6-one, CAS# 123548-16-3
• AFDX-384 (mixed M2/M4 antagonist) - N-[2-[2-[(Dipropylamino)methyl]-1-piperidinyl]ethyl]-5,6-dihydro-6-oxo-11H-pyrido[2,3-b][1,4]benzodiazepine-11-carboxamide, CAS# 118290-27-0
• dicycloverine^[11]
• Thorazine
• Diphenhydramine
• Dimenhydrinate
• tolterodine^[11]
• oxybutynin^[11]
• ipratropium^[11]
• methoctramine^[15]
• tripitramine
• gallamine
• chlorpromazine

See also

• Muscarinic acetylcholine receptor

References

1. "Drugs that physically interact with Muscarinic acetylcholine receptor M2 view/edit references on wikidata".
2. "Human PubMed Reference:".
3. "Mouse PubMed Reference:".
4. "Entrez Gene: CHRM2 cholinergic receptor, muscarinic 2".
5. Gosso MF, van Belzen M, de Geus EJ, Polderman JC, Heutink P, Boomsma DI, Posthuma D (November 2006). "Association between the CHRM2 gene and intelligence in a sample of 304 Dutch families". Genes Brain Behav. 5 (8): 577–84. doi:10.1111/j.1601-183X.2006.00211.x. PMID 17081262.
6. Comings DE, Wu S, Rostamkhani M, McGue M, Lacono WG, Cheng LS, MacMurray JP (January 2003). "Role of the cholinergic muscarinic 2 receptor (CHRM2) gene in cognition". Mol. Psychiatry. 8 (1): 10–1. doi:10.1038/sj.mp.4001095. PMID 12556901.
7. Dick DM, Aliev F, Kramer J, Wang JC, Hinrichs A, Bertelsen S, Kuperman S, Schuckit M, Nurnberger J, Edenberg HJ, Porjesz B, Begleiter H, Hesselbrock V, Goate A, Bierut L (March 2007). "Association of CHRM2 with IQ: converging evidence for a gene influencing intelligence". Behav. Genet. 37 (2): 265–72. doi:10.1007/s10519-006-9131-2. PMID 17160701.
8. Lind PA, Luciano M, Horan MA, Marioni RE, Wright MJ, Bates TC, Rabbitt P, Harris SE, Davidson Y, Deary IJ, Gibbons L, Pickles A, Ollier W, Pendleton N, Price JF, Payton A, Martin NG (September 2009). "No association between Cholinergic Muscarinic Receptor 2 (CHRM2) genetic variation and cognitive abilities in three independent samples". Behav. Genet. 39 (5): 513–23. doi:10.1007/s10519-009-9274-z. PMID 19418213.
9. Smith RS, Hu R, DeSouza A, Eberly CL, Krahe K, Chan W, Araneda RC (Jul 2015). "Differential Muscarinic Modulation in the Olfactory Bulb". The Journal of Neuroscience. 35 (30): 10773–85. doi:10.1523/JNEUROSCI.0099-15.2015. PMID 26224860.
10. Douglas CL, Baghdoyan HA, Lydic R (December 2001). "M2 muscarinic autoreceptors modulate acetylcholine release in prefrontal cortex of C57BL/6J mouse". J. Pharmacol. Exp. Ther. 299 (3): 960–6. PMID 11714883.
11. Rang HP (2003). Pharmacology. Edinburgh: Churchill Livingstone. ISBN 0-443-07145-4.
12. Boron, W. F & Boulpaep, E. L. (2005). Medical Physiology. Philadelphia: Elsevier Saunders. p. 387. ISBN 1-4160-2328-3.
13. Scapecchi S, Matucci R, Bellucci C, Buccioni M, Dei S, Guandalini L, Martelli C, Manetti D, Martini E, Marucci G, Nesi M, Romanelli MN, Teodori E, Gualtieri F (March 2006). "Highly chiral muscarinic ligands: the discovery of (2S,2'R,3'S,5'R)-1-methyl-2-(2-methyl-1,3-oxathiolan-5-yl)pyrrolidine 3-sulfoxide methyl iodide, a potent, functionally selective, M2 partial agonist". J. Med. Chem. 49 (6): 1925–31. doi:10.1021/jm0510878. PMID 16539379.
14. Edwards Pharmaceuticals, Inc.; Belcher Pharmaceuticals, Inc. (May 2010), "ED-SPAZ- hyoscyamine sulfate tablet, orally disintegrating", DailyMed, U.S. National Library of Medicine, retrieved January 13, 2013
15. Melchiorre C, Angeli P, Lambrecht G, Mutschler E, Picchio MT, Wess J (December 1987). "Antimuscarinic action of methoctramine, a new cardioselective M-2 muscarinic receptor antagonist, alone and in combination with atropine and gallamine". Eur. J. Pharmacol. 144 (2): 117–24. doi:10.1016/0014-2999(87)90509-7. PMID 3436364.

Further reading

• Goyal RK (1989). "Muscarinic receptor subtypes. Physiology and clinical implications.". N. Engl. J. Med. 321 (15): 1022–9. doi:10.1056/NEJM198910123211506. PMID 2674717.
• Brann MR, Ellis J, Jørgensen H, Hill-Eubanks D, Jones SV (1994). "Muscarinic acetylcholine receptor subtypes: localization and structure/function.". Prog. Brain Res. 98: 121–7. doi:10.1016/S0079-6123(08)62388-2. PMID 8248499.
• van Koppen CJ, Nathanson NM (1991). "Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor. Analysis of the role of N-glycosylation in receptor expression and function.". J. Biol. Chem. 265 (34): 20887–92. PMID 2249995.
• Ashkenazi A, Ramachandran J, Capon DJ (1989). "Acetylcholine analogue stimulates DNA synthesis in brain-derived cells via specific muscarinic receptor subtypes.". Nature. 340 (6229): 146–50. doi:10.1038/340146a0. PMID 2739737.
• Bonner TI, Buckley NJ, Young AC, Brann MR (1987). "Identification of a family of muscarinic acetylcholine receptor genes.". Science. 237 (4814): 527–32. doi:10.1126/science.3037705. PMID 3037705.
• Peralta EG, Ashkenazi A, Winslow JW, Smith DH, Ramachandran J, Capon DJ (1988). "Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.". EMBO J. 6 (13): 3923–9. PMC 553870. PMID 3443095.
• Badner JA, Yoon SW, Turner G, Bonner TI, Detera-Wadleigh SD (1995). "Multipoint genetic linkage analysis of the m2 human muscarinic receptor gene.". Mamm. Genome. 6 (7): 489–90. doi:10.1007/BF00360666. PMID 7579899.
• Offermanns S, Simon MI (1995). "G alpha 15 and G alpha 16 couple a wide variety of receptors to phospholipase C.". J. Biol. Chem. 270 (25): 15175–80. doi:10.1074/jbc.270.25.15175. PMID 7797501.
• Russell M, Winitz S, Johnson GL (1994). "Acetylcholine muscarinic m1 receptor regulation of cyclic AMP synthesis controls growth factor stimulation of Raf activity.". Mol. Cell. Biol. 14 (4): 2343–51. doi:10.1128/mcb.14.4.2343. PMC 358601. PMID 8139539.
• Kunapuli P, Onorato JJ, Hosey MM, Benovic JL (1994). "Expression, purification, and characterization of the G protein-coupled receptor kinase GRK5.". J. Biol. Chem. 269 (2): 1099–105. PMID 8288567.
• Haga K, Kameyama K, Haga T, Kikkawa U, Shiozaki K, Uchiyama H (1996). "Phosphorylation of human m1 muscarinic acetylcholine receptors by G protein-coupled receptor kinase 2 and protein kinase C.". J. Biol. Chem. 271 (5): 2776–82. doi:10.1074/jbc.271.5.2776. PMID 8576254.
• Kostenis E, Conklin BR, Wess J (1997). "Molecular basis of receptor/G protein coupling selectivity studied by coexpression of wild type and mutant m2 muscarinic receptors with mutant G alpha(q) subunits.". Biochemistry. 36 (6): 1487–95. doi:10.1021/bi962554d. PMID 9063897.
• Smiley JF, Levey AI, Mesulam MM (1998). "Infracortical interstitial cells concurrently expressing m2-muscarinic receptors, acetylcholinesterase and nicotinamide adenine dinucleotide phosphate-diaphorase in the human and monkey cerebral cortex.". Neuroscience. 84 (3): 755–69. doi:10.1016/S0306-4522(97)00524-1. PMID 9579781.
• von der Kammer H, Mayhaus M, Albrecht C, Enderich J, Wegner M, Nitsch RM (1998). "Muscarinic acetylcholine receptors activate expression of the EGR gene family of transcription factors.". J. Biol. Chem. 273 (23): 14538–44. doi:10.1074/jbc.273.23.14538. PMID 9603968.
• Sato KZ, Fujii T, Watanabe Y, Yamada S, Ando T, Kazuko F, Kawashima K (1999). "Diversity of mRNA expression for muscarinic acetylcholine receptor subtypes and neuronal nicotinic acetylcholine receptor subunits in human mononuclear leukocytes and leukemic cell lines.". Neurosci. Lett. 266 (1): 17–20. doi:10.1016/S0304-3940(99)00259-1. PMID 10336173.
• Retondaro FC, Dos Santos Costa PC, Pedrosa RC, Kurtenbach E (1999). "Presence of antibodies against the third intracellular loop of the m2 muscarinic receptor in the sera of chronic chagasic patients.". FASEB J. 13 (14): 2015–20. PMID 10544184.
• Waid DK, Chell M, El-Fakahany EE (2000). "M(2) and M(4) muscarinic receptor subtypes couple to activation of endothelial nitric oxide synthase.". Pharmacology. 61 (1): 37–42. doi:10.1159/000028378. PMID 10895079.
• Obara K, Arai K, Miyajima N, Hatano A, Tomita Y, Takahashi K (2000). "Expression of m2 muscarinic acetylcholine receptor mRNA in primary culture of human prostate stromal cells.". Urol. Res. 28 (3): 196–200. doi:10.1007/s002400000113. PMID 10929429.
• Matera C, Flammini L, Quadri M, Vivo V, Ballabeni V, Holzgrabe U, Mohr K, De Amici M, Barocelli E, Bertoni S, Dallanoce C (2014). "Bis(ammonio)alkane-type agonists of muscarinic acetylcholine receptors: synthesis, in vitro functional characterization, and in vivo evaluation of their analgesic activity.". Eur. J. Med. Chem. 75: 222–232. doi:10.1016/j.ejmech.2014.01.032. PMID 24534538.

External links

• "Acetylcholine receptors (muscarinic): M₂". IUPHAR Database of Receptors and Ion Channels. International Union of Basic and Clinical Pharmacology.
• CHRM2 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.