Dynein

Cytoplasmic dynein on a microtubule
Cytoplasmic dynein has two heavy chains with globular "heads" that "walk" along the microtubule, to which they are bound by the "stalks". Dynactin (not shown) may help attach the light chains to the cargo. Interactions between the "stalks" and the microtubule must repeatedly form and break (see main text for details)

Dynein is a family of cytoskeletal motor proteins that move along microtubules in cells. They convert the chemical energy stored in ATP to mechanical work. Dynein transports various cellular cargos, provides forces and displacements important in mitosis, and drives the beat of eukaryotic cilia and flagella. All of these functions rely on dynein's ability to move towards the minus-end of the microtubules, known as retrograde transport, thus, they are called "minus-end directed motors." In contrast, kinesin motor proteins move toward the microtubules' plus end.

Classification

Dynein heavy chain, N-terminal region 1
Identifiers
Symbol DHC_N1
Pfam PF08385
InterPro IPR013594
Dynein heavy chain, N-terminal region 2
Identifiers
Symbol DHC_N2
Pfam PF08393
InterPro IPR013602
Dynein heavy chain and region D6 of dynein motor
Identifiers
Symbol Dynein_heavy
Pfam PF03028
InterPro IPR004273
Dynein light intermediate chain (DLIC)
Identifiers
Symbol DLIC
Pfam PF05783
Pfam clan CL0023
Dynein light chain type 1

structure of the human pin/lc8 dimer with a bound peptide
Identifiers
Symbol Dynein_light
Pfam PF01221
InterPro IPR001372
PROSITE PDOC00953
SCOP 1bkq
SUPERFAMILY 1bkq

Dyneins can be divided into two groups: cytoplasmic dyneins and axonemal dyneins, which are also called ciliary or flagellar dyneins.

Function

Axonemal dynein causes sliding of microtubules in the axonemes of cilia and flagella and is found only in cells that have those structures.

Cytoplasmic dynein, found in all animal cells and possibly plant cells as well, performs functions necessary for cell survival such as organelle transport and centrosome assembly.[1] Cytoplasmic dynein moves processively along the microtubule; that is, one or the other of its stalks is always attached to the microtubule so that the dynein can "walk" a considerable distance along a microtubule without detaching.

Cytoplasmic dynein helps to position the Golgi complex and other organelles in the cell.[1] It also helps transport cargo needed for cell function such as vesicles made by the endoplasmic reticulum, endosomes, and lysosomes (Karp, 2005). Dynein is involved in the movement of chromosomes and positioning the mitotic spindles for cell division.[2][3] Dynein carries organelles, vesicles and possibly microtubule fragments along the axons of neurons toward the cell body in a process called retrograde axoplasmic transport.[1]

Mitotic Spindle Positioning

Cytoplasmic dynein positions the spindle at the site of cytokinesis by anchoring to the cell cortex and pulling on astral microtubules emanating from centrosome.[4][5][6] Budding yeast have been a powerful model organism to study this process and has shown that dynein is targeted to plus ends of astral microtubules and delivered to the cell cortex via an offloading mechanism.[7][8]

Structure

Each molecule of the dynein motor is a complex protein assembly composed of many smaller polypeptide subunits. Cytoplasmic and axonemal dynein contain some of the same components, but they also contain some unique subunits

Cytoplasmic dynein

Cytoplasmic dynein, which has a molecular mass of about 1.5 megadaltons (MDa), is a dimer of dimers, containing approximately twelve polypeptide subunits: two identical "heavy chains", 520 kDa in mass, which contain the ATPase activity and are thus responsible for generating movement along the microtubule; two 74 kDa intermediate chains which are believed to anchor the dynein to its cargo; two 53–59 kDa light intermediate chains; and several light chains..

The force-generating ATPase activity of each dynein heavy chain is located in its large doughnut-shaped "head", which is related to other AAA proteins, while two projections from the head connect it to other cytoplasmic structures. One projection, the coiled-coil stalk, binds to and "walks" along the surface of the microtubule via a repeated cycle of detachment and reattachment. The other projection, the extended tail, binds to the light intermediate, intermediate and light chain subunits which attach dynein to its cargo. The alternating activity of the paired heavy chains in the complete cytoplasmic dynein motor enables a single dynein molecule to transport its cargo by "walking" a considerable distance along a microtubule without becoming completely detached.

Yeast dynein can walk along microtubules without detaching, however in metazoans, cytoplasmic dynein must be activated by the binding of dynactin, another multisubunit protein that is essential for mitosis, and a cargo adaptor.[9] The tri-complex, which includes dynein, dynactin and a cargo adaptor, is ultra-processive and can walk long distances without detaching in order to reach the cargo's intracellular destination. Cargo adaptors identified thus far include BicD2, Hook3, FIP3and Spindly.[9] The light intermediate chain, which is a member of the Ras superfamily, mediates the attachment of several cargo adaptors to the dynein motor.[10] The other tail subunits may also help facilitate this interaction as evidenced in a low resolution structure of dynein-dynactin-BicD2.[11]

Axonemal dynein

A cross-section of an axoneme, with axonemal dynein arms

Axonemal dyneins come in multiple forms that contain either one, two or three non-identical heavy chains (depending upon the organism and location in the cilium). Each heavy chain has a globular motor domain with a doughnut-shaped structure believed to resemble that of other AAA proteins, a coiled coil "stalk" that binds to the microtubule, and an extended tail (or "stem") that attaches to a neighboring microtubule of the same axoneme. Each dynein molecule thus forms a cross-bridge between two adjacent microtubules of the ciliary axoneme. During the "power stroke", which causes movement, the AAA ATPase motor domain undergoes a conformational change that causes the microtubule-binding stalk to pivot relative to the cargo-binding tail with the result that one microtubule slides relative to the other (Karp, 2005). This sliding produces the bending movement needed for cilia to beat and propel the cell or other particles. Groups of dynein molecules responsible for movement in opposite directions are probably activated and inactivated in a coordinated fashion so that the cilia or flagella can move back and forth. The radial spoke has been proposed as the (or one of the) structures that synchronizes this movement.

History

The protein responsible for movement of cilia and flagella was first discovered and named dynein in 1963 (Karp, 2005). 20 years later, cytoplasmic dynein, which had been suspected to exist since the discovery of flagellar dynein, was isolated and identified (Karp, 2005).

Chromosome segregation during meiosis

Segregation of homologous chromosomes to opposite poles of the cell occurs during the first division of meiosis. Proper segregation is essential for producing haploid meiotic products with a normal complement of chromosomes. The formation of chiasmata (crossover recombination events) appears to generally facilitate proper segregation. However, in the fission yeast Schizosaccharomyces pombe, when chiasmata are absent, dynein promotes segregation.[12] Dhc1, the motor subunit of dynein, is required for chromosomal segregation in both the presence and absence of chiasmata.[12] The dynein light chain Dlc1 protein is also required for segregation, specifically when chiasmata are absent.

See also

References

  1. 1 2 3 Gerald Karp; Kurt Beginnen; Sebastian Vogel; Susanne Kuhlmann-Krieg (2005). Molekulare Zellbiologie (in French). Springer. ISBN 978-3-540-23857-7.
  2. ↑ Samora, CP; Mogessie, B; Conway, L; Ross, JL; Straube, A; McAinsh, AD (Aug 7, 2011). "MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis.". Nature Cell Biology. 13 (9): 1040–50. doi:10.1038/ncb2297. PMID 21822276.
  3. ↑ Kiyomitsu, Tomomi; Iain M. Cheeseman (2012-02-12). "Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation". Nature Cell Biology. doi:10.1038/ncb2440. ISSN 1465-7392. Retrieved 2012-02-14.
  4. ↑ Eshel, D.; Urrestarazu, L. A.; Vissers, S.; Jauniaux, J. C.; van Vliet-Reedijk, J. C.; Planta, R. J.; Gibbons, I. R. (1993-12-01). "Cytoplasmic dynein is required for normal nuclear segregation in yeast". Proceedings of the National Academy of Sciences of the United States of America. 90 (23): 11172–11176. doi:10.1073/pnas.90.23.11172. ISSN 0027-8424. PMC 47944Freely accessible. PMID 8248224.
  5. ↑ Li, Y. Y.; Yeh, E.; Hays, T.; Bloom, K. (1993-11-01). "Disruption of mitotic spindle orientation in a yeast dynein mutant". Proceedings of the National Academy of Sciences of the United States of America. 90 (21): 10096–10100. doi:10.1073/pnas.90.21.10096. ISSN 0027-8424. PMC 47720Freely accessible. PMID 8234262.
  6. ↑ Carminati, J. L.; Stearns, T. (1997-08-11). "Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex". The Journal of Cell Biology. 138 (3): 629–641. doi:10.1083/jcb.138.3.629. ISSN 0021-9525. PMC 2141630Freely accessible. PMID 9245791.
  7. ↑ Lee, Wei-Lih; Oberle, Jessica R.; Cooper, John A. (2003-02-03). "The role of the lissencephaly protein Pac1 during nuclear migration in budding yeast". The Journal of Cell Biology. 160 (3): 355–364. doi:10.1083/jcb.200209022. ISSN 0021-9525. PMC 2172672Freely accessible. PMID 12566428.
  8. ↑ Lee, Wei-Lih; Kaiser, Michelle A.; Cooper, John A. (2005-01-17). "The offloading model for dynein function: differential function of motor subunits". The Journal of Cell Biology. 168 (2): 201–207. doi:10.1083/jcb.200407036. ISSN 0021-9525. PMC 2171595Freely accessible. PMID 15642746.
  9. 1 2 McKenney, Richard J.; Huynh, Walter; Tanenbaum, Marvin E.; Bhabha, Gira; Vale, Ronald D. (2014-07-18). "Activation of cytoplasmic dynein motility by dynactin-cargo adapter complexes". Science. 345 (6194): 337–341. doi:10.1126/science.1254198. ISSN 0036-8075. PMC 4224444Freely accessible. PMID 25035494.
  10. ↑ Schroeder, Courtney M.; Ostrem, Jonathan ML; Hertz, Nicholas T.; Vale, Ronald D. (2014-10-01). "A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region". eLife. 3: e03351. doi:10.7554/eLife.03351. ISSN 2050-084X. PMC 4359372Freely accessible. PMID 25272277.
  11. ↑ Urnavicius, Linas; Zhang, Kai; Diamant, Aristides G.; Motz, Carina; Schlager, Max A.; Yu, Minmin; Patel, Nisha A.; Robinson, Carol V.; Carter, Andrew P. (2015-03-27). "The structure of the dynactin complex and its interaction with dynein". Science. 347 (6229): 1441–1446. doi:10.1126/science.aaa4080. ISSN 0036-8075. PMC 4413427Freely accessible. PMID 25814576.
  12. 1 2 Davis L, Smith GR (2005). "Dynein promotes achiasmate segregation in Schizosaccharomyces pombe". Genetics. 170 (2): 581–90. doi:10.1534/genetics.104.040253. PMC 1450395Freely accessible. PMID 15802518.

External links

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