IRF3

IRF3
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3QU6, 1J2F, 1QWT, 1T2K, 1ZOQ, 2O61, 2O6G, 2PI0, 3A77, 5JEO, 5JEK, 5JER, 5JEM, 5JEL, 5JEJ
Identifiers
Aliases	IRF3, entrez:3661, IIAE7, interferon regulatory factor 3
External IDs	OMIM: 603734 MGI: 1859179 HomoloGene: 1208 GeneCards: IRF3
Gene ontology Molecular function • DNA binding • protein homodimerization activity • transcription factor activity, sequence-specific DNA binding • transcription cofactor activity • regulatory region DNA binding • RNA polymerase II core promoter proximal region sequence-specific DNA binding • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding • protein binding • identical protein binding Cellular component • cytoplasm • cytosol • nucleoplasm • nucleus Biological process • apoptotic process • positive regulation of type I interferon-mediated signaling pathway • macrophage apoptotic process • regulation of transcription, DNA-templated • response to bacterium • response to exogenous dsRNA • positive regulation of interferon-alpha production • MDA-5 signaling pathway • interferon-gamma-mediated signaling pathway • immune system process • positive regulation of cytokine secretion • negative regulation of transcription from RNA polymerase II promoter • transcription from RNA polymerase II promoter • cellular response to dsRNA • transcription, DNA-templated • cellular response to DNA damage stimulus • TRIF-dependent toll-like receptor signaling pathway • response to lipopolysaccharide • defense response to virus • type I interferon signaling pathway • type I interferon biosynthetic process • positive regulation of I-kappaB kinase/NF-kappaB signaling • negative regulation of type I interferon production • programmed necrotic cell death • viral process • cellular response to lipopolysaccharide • positive regulation of type I interferon production • lipopolysaccharide-mediated signaling pathway • positive regulation of interferon-beta production • innate immune response Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	3661	54131
Ensembl	ENSG00000126456	ENSMUSG00000003184
UniProt	Q14653	P70671
RefSeq (mRNA)	NM_001197122 NM_001197123 NM_001197124 NM_001197125 NM_001197126 NM_001197127 NM_001197128 NM_001571	NM_016849
RefSeq (protein)	NP_001184051.1 NP_001184052.1 NP_001184053.1 NP_001184054.1 NP_001184055.1 NP_001184056.1 NP_001184057.1 NP_001562.1 NP_001184053.1 NP_001184056.1 NP_001184057.1	NP_058545.1
Location (UCSC)	Chr 19: 49.66 – 49.67 Mb	Chr 7: 45 – 45 Mb
PubMed search	[1]	[2]
Wikidata

View/Edit Human

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Interferon regulatory factor 3, also known as IRF3, is an interferon regulatory factor.^[3]

Function

IRF3 is a member of the interferon regulatory transcription factor (IRF) family.^[3] IRF3 was originally discovered as a homolog of IRF1 and IRF2. IRF3 has been further characterized and shown to contain several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain and several regulatory phosphorylation sites.^[4] IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP.^[5] This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.^[6]

IRF3 plays an important role in the innate immune system's response to viral infection.^[7] Aggregated MAVS have been found to activate IRF3 dimerization.^[8] A recent study shows phosphorylation of innate immune adaptor proteins MAVS, STING and TRIF at a conserved pLxIS motif recruits and specifies IRF3 phosphorylation and activation by the Serine/threonine-protein kinase TBK1, thereby restricts the production of type-I interferons.^[9]

Signaling pathway of toll-like receptors. Dashed grey lines represent unknown associations

Interactions

IRF3 has been shown to interact with IRF7.^[10]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Hiscott J, Pitha P, Genin P, Nguyen H, Heylbroeck C, Mamane Y, Algarte M, Lin R (1999). "Triggering the interferon response: the role of IRF-3 transcription factor". J. Interferon Cytokine Res. 19 (1): 1–13. doi:10.1089/107999099314360. PMID 10048763.
4. Lin R, Heylbroeck C, Genin P, Pitha PM, Hiscott J (Feb 1999). "Essential Role of Interferon Regulatory Factor 3 in Direct Activation of RANTES Chemokine Transcription". Mol Cell Biol. 19 (2): 959–66. PMC 116027. PMID 9891032.
5. Yoneyama M, Suhara W, Fujita T (2002). "Control of IRF-3 activation by phosphorylation". J. Interferon Cytokine Res. 22 (1): 73–6. doi:10.1089/107999002753452674. PMID 11846977.
6. "Entrez Gene: IRF3 interferon regulatory factor 3".
7. Collins SE, Noyce RS, Mossman KL (Feb 2004). "Innate Cellular Response to Virus Particle Entry Requires IRF3 but Not Virus Replication". J Virol. 78 (4): 1706–17. doi:10.1128/JVI.78.4.1706-1717.2004. PMC 369475. PMID 14747536.
8. Hou F, Sun L, Zheng H, Skaug B, Jiang QX, Chen ZJ (Aug 5, 2011). "MAVS Forms Functional Prion-Like Aggregates To Activate and Propagate Antiviral Innate Immune Response". Cell. 146 (3): 448–61. doi:10.1016/j.cell.2011.06.041. PMC 3179916. PMID 21782231.
9. Liu S, Cai X, Wu J, Cong Q, Chen X, Li T, Du F, Ren J, Wu Y, Grishin N, and Chen ZJ (Mar 13, 2015). "Phosphorylation of innate immune adaptor proteins MAVS, STING, and TRIF induces IRF3 activation.". Science. 347 (6227): aaa2630. doi:10.1126/science.aaa2630. PMID 25636800.
10. Au WC, Yeow WS, Pitha PM (Feb 2001). "Analysis of functional domains of interferon regulatory factor 7 and its association with IRF-3". Virology. 280 (2): 273–82. doi:10.1006/viro.2000.0782. PMID 11162841.

Further reading

• Pitha PM, Au WC, Lowther W, Juang YT, Schafer SL, Burysek L, Hiscott J, Moore PA (1999). "Role of the interferon regulatory factors (IRFs) in virus-mediated signaling and regulation of cell growth". Biochimie. 80 (8–9): 651–8. doi:10.1016/S0300-9084(99)80018-2. PMID 9865487.
• Yoneyama M, Suhara W, Fujita T (2002). "Control of IRF-3 activation by phosphorylation". J. Interferon Cytokine Res. 22 (1): 73–6. doi:10.1089/107999002753452674. PMID 11846977.
• Au WC, Moore PA, Lowther W, Juang YT, Pitha PM (1996). "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes". Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11657–61. doi:10.1073/pnas.92.25.11657. PMC 40461. PMID 8524823.
• Yoneyama M, Suhara W, Fukuhara Y, Fukuda M, Nishida E, Fujita T (1998). "Direct triggering of the type I interferon system by virus infection: activation of a transcription factor complex containing IRF-3 and CBP/p300". EMBO J. 17 (4): 1087–95. doi:10.1093/emboj/17.4.1087. PMC 1170457. PMID 9463386.
• Weaver BK, Kumar KP, Reich NC (1998). "Interferon Regulatory Factor 3 and CREB-Binding Protein/p300 Are Subunits of Double-Stranded RNA-Activated Transcription Factor DRAF1". Mol. Cell. Biol. 18 (3): 1359–68. PMC 108849. PMID 9488451.
• Lin R, Heylbroeck C, Pitha PM, Hiscott J (1998). "Virus-Dependent Phosphorylation of the IRF-3 Transcription Factor Regulates Nuclear Translocation, Transactivation Potential, and Proteasome-Mediated Degradation". Mol. Cell. Biol. 18 (5): 2986–96. PMC 110678. PMID 9566918.
• Ronco LV, Karpova AY, Vidal M, Howley PM (1998). "Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity". Genes Dev. 12 (13): 2061–72. doi:10.1101/gad.12.13.2061. PMC 316980. PMID 9649509.
• Bellingham J, Gregory-Evans K, Gregory-Evans CY (1999). "Mapping of human interferon regulatory factor 3 (IRF3) to chromosome 19q13.3-13.4 by an intragenic polymorphic marker". Annals of Human Genetics. 62 (Pt 3): 231–4. doi:10.1046/j.1469-1809.1998.6230231.x. PMID 9803267.
• Lowther WJ, Moore PA, Carter KC, Pitha PM (1999). "Cloning and functional analysis of the human IRF-3 promoter". DNA Cell Biol. 18 (9): 685–92. doi:10.1089/104454999314962. PMID 10492399.
• Kim T, Kim TY, Song YH, Min IM, Yim J, Kim TK (1999). "Activation of interferon regulatory factor 3 in response to DNA-damaging agents". J. Biol. Chem. 274 (43): 30686–9. doi:10.1074/jbc.274.43.30686. PMID 10521456.
• Kumar KP, McBride KM, Weaver BK, Dingwall C, Reich NC (2000). "Regulated Nuclear-Cytoplasmic Localization of Interferon Regulatory Factor 3, a Subunit of Double-Stranded RNA-Activated Factor 1". Mol. Cell. Biol. 20 (11): 4159–68. doi:10.1128/MCB.20.11.4159-4168.2000. PMC 85785. PMID 10805757.
• Suhara W, Yoneyama M, Iwamura T, Yoshimura S, Tamura K, Namiki H, Aimoto S, Fujita T (2000). "Analyses of virus-induced homomeric and heteromeric protein associations between IRF-3 and coactivator CBP/p300". J. Biochem. 128 (2): 301–7. doi:10.1093/oxfordjournals.jbchem.a022753. PMID 10920266.
• Servant MJ, ten Oever B, LePage C, Conti L, Gessani S, Julkunen I, Lin R, Hiscott J (2001). "Identification of distinct signaling pathways leading to the phosphorylation of interferon regulatory factor 3". J. Biol. Chem. 276 (1): 355–63. doi:10.1074/jbc.M007790200. PMID 11035028.
• Smith EJ, Marié I, Prakash A, García-Sastre A, Levy DE (2001). "IRF3 and IRF7 phosphorylation in virus-infected cells does not require double-stranded RNA-dependent protein kinase R or Ikappa B kinase but is blocked by Vaccinia virus E3L protein". J. Biol. Chem. 276 (12): 8951–7. doi:10.1074/jbc.M008717200. PMID 11124948.
• Au WC, Yeow WS, Pitha PM (2001). "Analysis of functional domains of interferon regulatory factor 7 and its association with IRF-3". Virology. 280 (2): 273–82. doi:10.1006/viro.2000.0782. PMID 11162841.
• Barnes BJ, Moore PA, Pitha PM (2001). "Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes". J. Biol. Chem. 276 (26): 23382–90. doi:10.1074/jbc.M101216200. PMID 11303025.
• Mach CM, Hargrove BW, Kunkel GR (2002). "The Small RNA gene activator protein, SphI postoctamer homology-binding factor/selenocysteine tRNA gene transcription activating factor, stimulates transcription of the human interferon regulatory factor-3 gene". J. Biol. Chem. 277 (7): 4853–8. doi:10.1074/jbc.M108308200. PMID 11724783.
• Morin P, Bragança J, Bandu MT, Lin R, Hiscott J, Doly J, Civas A (2002). "Preferential binding sites for interferon regulatory factors 3 and 7 involved in interferon-A gene transcription". J. Mol. Biol. 316 (5): 1009–22. doi:10.1006/jmbi.2001.5401. PMID 11884139.
• Dang O, Navarro L, Anderson K, David M (2004). "Cutting edge: anthrax lethal toxin inhibits activation of IFN-regulatory factor 3 by lipopolysaccharide". J. Immunology. 172 (2): 747–51. doi:10.4049/jimmunol.172.2.747. PMID 14707042.

External links

• Interferon Regulatory Factor-3 at the US National Library of Medicine Medical Subject Headings (MeSH)
• FactorBook IRF3